Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics

Innate lymphoid cells (ILCs) are key components of the immune system, yet the similarity and distinction of the properties across tissues under homeostasis, inflammation and tumor process remain elusive. Here we performed integrative inference of ILCs to reveal their transcriptional profiles and het...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in immunology 2023-03, Vol.14, p.1127413-1127413
Main Authors: Song, Peng, Cao, Ke, Mao, Yonghuan, Ai, Shichao, Sun, Feng, Hu, Qiongyuan, Liu, Song, Wang, Meng, Lu, Xiaofeng, Guan, Wenxian, Shen, Xiaofei
Format: Article
Language:English
Subjects:
cell heterogeneity
Homeostasis
Humans
Immunity, Innate
Immunology
Inflammation
innate lymphoid cells
integrative inference
Lymphocytes
single-cell transcriptomics
tissue imprinting
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Innate lymphoid cells (ILCs) are key components of the immune system, yet the similarity and distinction of the properties across tissues under homeostasis, inflammation and tumor process remain elusive. Here we performed integrative inference of ILCs to reveal their transcriptional profiles and heterogeneity from single-cell genomics. We collected a large number of ILCs from human six different tissues which can represent unique immune niches (circulation, lymphoid tissue, normal and inflamed mucosa, tumor microenvironment), to systematically address the transcriptional imprinting. ILCs are profoundly imprinted by their organ of residence, and tissue-specific distinctions are apparent under pathological conditions. In the hepatocellular carcinoma microenvironment, we identified intermediate c-kit ILC2 population, and lin CD127 NK-like cells that expressed markers of cytotoxicity including and . Additionally, CD127 CD94 ILC1s were preferentially enriched in inflamed ileum from patients with Crohn's disease. These analyses depicted a comprehensive characterization of ILC anatomical distribution and subset heterogeneity, and provided a base line for future temporal or spatial studies focused on tissue-specific ILC-mediated immunity.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2023.1127413