An atypical phosphodiesterase capable of degrading haloalkyl phosphate diesters from Sphingobium sp. strain TCM1

• Published in: Scientific reports 2017-06, Vol.7 (1), p.2842-9, Article 2842
• Main Authors: Abe, Katsumasa, Mukai, Naoko, Morooka, Yuka, Makino, Takeshi, Oshima, Kenji, Takahashi, Shouji, Kera, Yoshio
• Format: Article
• Language: English
• Subjects: 45; 631/326/171/1818; 631/45/607/1164; 82/80; 82/83; Amino Acid Sequence; Enzymes; Humanities and Social Sciences; Hydrogen-Ion Concentration; Hydrolysis; multidisciplinary; p-Nitrophenylphosphate; Phosphate; Phosphates; Phosphates - metabolism; Phosphodiesterase; Phosphoric Triester Hydrolases - chemistry; Phosphoric Triester Hydrolases - metabolism; Phosphotriesterase; Science; Science (multidisciplinary); Sphingobium; Sphingomonadaceae - metabolism; Temperature
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-03142-9

Sphingobium sp. strain TCM1 can degrade tris(2-chloroethyl) phosphate (TCEP) to inorganic phosphate and 2-chloroethanol. A phosphotriesterase (PTE), phosphodiesterase (PDE) and phosphomonoesterase (PME) are believed to be involved in the degradation of TCEP. The PTE and PME that respectively catalyze the first and third steps of TCEP degradation in TCM1 have been identified. However, no information has been reported on a PDE catalyzing the second step. In this study, we identified, purified, and characterized a PDE capable of hydrolyzing haloalkyl phosphate diesters. The final preparation of the enzyme had a specific activity of 29 µmol min −1  mg −1 with bis( p -nitrophenyl) phosphate (B p NPP) as the substrate. It also possessed low PME activity with p -nitrophenyl phosphate ( p NPP) as substrate. The catalytic efficiency ( k cat / K m ) with B p NPP was significantly higher than that with p NPP, indicating that the enzyme prefers the organophosphorus diester to the monoester. The enzyme degraded bis(2,3-dibromopropyl) phosphate, bis(1,3-dichloro-2-propyl) phosphate and bis(2-chloroethyl) phosphate, suggesting that it is involved in the metabolism of haloalkyl organophosphorus triesters. The primary structure of the PDE from TCM1 is distinct from those of typical PDE family members and the enzyme belongs to the polymerase and histidinol phosphatase superfamily.