Quantitative analysis of MBW complex formation in the context of trichome patterning

Trichome patterning in is regulated by , and (MBW) genes. These are considered to form a trimeric MBW protein complex that promotes trichome formation. The MBW proteins are engaged in a regulatory network to select trichome cells among epidermal cells through R3MYB proteins that can move between cel...

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Bibliographic Details
Published in:Frontiers in plant science 2024-03, Vol.15, p.1331156-1331156
Main Authors: Zhang, Bipei, Deneer, Anna, Fleck, Christian, Hülskamp, Martin
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
dissociation constant
mathematical modelling
Plant Science
protein complex formation
protein interactions
trichome patterning
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Summary:Trichome patterning in is regulated by , and (MBW) genes. These are considered to form a trimeric MBW protein complex that promotes trichome formation. The MBW proteins are engaged in a regulatory network to select trichome cells among epidermal cells through R3MYB proteins that can move between cells and repress the MBW complex by competitive binding with the R2R3MYB to the bHLHL protein. We use quantitative pull-down assays to determine the relative dissociation constants for the protein-protein interactions of the involved genes. We find similar binding strength between the trichome promoting genes and weaker binding of the R3MYB inhibitors. We used the dissociation constants to calculate the relative percentage of all possible complex combinations and found surprisingly low fractions of those complexes that are typically considered to be relevant for the regulation events. Finally, we predict an increased robustness in patterning as a consequence of higher ordered complexes mediated by GL3 dimerization.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2024.1331156