Prognostic Significance of TLR2, SMAD3 and Localization-dependent SATB1 in Stage I and II Non–Small-Cell Lung Cancer Patients

• Published in: Cancer control 2021-01, Vol.28, p.10732748211056697-10732748211056697
• Main Authors: Durślewicz, Justyna, Klimaszewska-Wiśniewska, Anna, Jóźwicki, Jakub, Antosik, Paulina, Smolińska-Świtała, Marta, Gagat, Maciej, Kowalewski, Adam, Grzanka, Dariusz
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Biomarkers; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - mortality; Carcinoma, Non-Small-Cell Lung - pathology; Female; Gene expression; Genomes; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Localization; Lung cancer; Lung carcinoma; Lung Neoplasms - genetics; Lung Neoplasms - mortality; Lung Neoplasms - pathology; Male; Matrix Attachment Region Binding Proteins - analysis; Medical prognosis; Middle Aged; Neoplasm Staging; Non-small cell lung carcinoma; Original; Patients; Prognosis; Smad3 protein; Smad3 Protein - analysis; Small cell lung carcinoma; Survival Rate; TLR2 protein; Toll-Like Receptor 2 - analysis; Toll-like receptors
• ISSN: 1073-2748; 1526-2359; 1073-2748
• DOI: 10.1177/10732748211056697

This study aimed to explore the prognostic value of SATB1, SMAD3, and TLR2 expression in non–small-cell lung carcinoma patients with clinical stages I-II. To investigate, we evaluated immunohistochemical staining to each of these markers using tissue sections from 69 patients from our cohort and gene expression data for The Cancer Genome Atlas (TCGA) cohort. We found that, in our cohort, high expression levels of nuclear SATB1n and SMAD3 were independent prognostic markers for better overall survival (OS) in NSCLC patients. Interestingly, expression of cytoplasmic SATB1c exhibited a significant but inverse association with survival rate, and it was an independent predictor of unfavorable prognosis. Likewise, TLR2 was a negative outcome biomarker for NSCLC even when adjusting for covariates. Importantly, stratification of NSCLCs with respect to combined expression of the three biomarkers allowed us to identify subgroups of patients with the greatest difference in duration of survival. Specifically, expression profile of SATB1n-high/SMAD3high/TLR2low was associated with the best OS, and it was superior to each single protein alone in predicting patient prognosis. Furthermore, based on the TCGA dataset, we found that overexpression of SATB1 mRNA was significantly associated with better OS, whereas high mRNA levels of SMAD3 and TLR2 with poor OS. In conclusion, the present study identified a set of proteins that may play a significant role in predicting prognosis of NSCLC patients with clinical stages I-II.