Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, Pardosa pseudoannulata

Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arth...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-10, Vol.22 (21), p.11721
Main Authors: Yang, Zhi-Ming, Wu, Yong, Li, Fang-Fang, Zhou, Zhang-Jin, Yu, Na, Liu, Ze-Wen
Format: Article
Language:English
Subjects:
Abdomen
Amino acids
Animals
Arthropod Proteins - genetics
Arthropod Proteins - metabolism
Arthropoda
Arthropods
Biosynthesis
Body weight
Chromosome 1
Chromosome 13
Chromosomes
Eggs
Enzymes
Female
Functional analysis
gene expansion
Gene Expression
Genes
Genomes
Insects
JHAMT
Juvenile hormones
Juvenile Hormones - genetics
Juvenile Hormones - metabolism
Male
Males
Methionine
Methyltransferase
Methyltransferases - genetics
Methyltransferases - metabolism
Molting
Pardosa pseudoannulata
Phylogenetics
Phylogeny
Ponds
RNA interference
Spiders
Spiders - genetics
Spiders - metabolism
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Summary:Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, , all containing the well conserved S-adenosyl-L-methionine binding motif. and the other seven were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, , , and were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of in Arachnida.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms222111721