High similarity of Trypanosoma cruzi kDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil

Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease. An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic ind...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 2014-09, Vol.47 (5), p.653-656
Main Authors: Alkmim-Oliveira, Sandra Maria, Kappel, Henrique Borges, Andrade, Cristiane Pontes, Prata, Aluízio, Ramirez, Luis Eduardo, Correia, Dalmo, Lages-Silva, Eliane
Format: Article
Language:English
Subjects:
Adolescent
Adult
Brazil - epidemiology
Chagas Disease - epidemiology
Chagas Disease - parasitology
Child
Child, Preschool
DNA, Kinetoplast - genetics
DNA, Protozoan
Endemic area
Female
Genetic Variation
Genotype
Humans
LSSP-PCR
Male
Middle Aged
Polymerase Chain Reaction
Trypanosoma cruzi
Trypanosoma cruzi - genetics
Trypanosoma cruzi - isolation & purification
Young Adult
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Summary:Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease. An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture). T. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected. LSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease.
ISSN:1678-9849
1678-9849
DOI:10.1590/0037-8682-0255-2013