Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method

In recent years, the wine industry has shown a considerable degree of interest in the occurrence of melatonin in wines. Sample pretreatment may be the most important step in trace analysis. Since wine is a complex matrix and melatonin is present in low amounts (ppb), an adequate extraction technique...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-03, Vol.28 (6), p.2768
Main Authors: Eremia, Sandra A V, Albu, Camelia, Radu, Gabriel L, Ion, Marian
Format: Article
Language:English
Subjects:
Antioxidants
Calibration
Chromatography, High Pressure Liquid - methods
DLLME
Efficiency
Enzymes
Fluorescence
High performance liquid chromatography
HPLC-FL
Liquid chromatography
Melatonin
Melatonin - analysis
Methods
Quantitative analysis
QuEChERS
Reproducibility
Retention
Sensors
Solid Phase Extraction - methods
Solid phases
SPE
Wine
Wine - analysis
Wine industry
Wineries
Wines
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Summary:In recent years, the wine industry has shown a considerable degree of interest in the occurrence of melatonin in wines. Sample pretreatment may be the most important step in trace analysis. Since wine is a complex matrix and melatonin is present in low amounts (ppb), an adequate extraction technique is required. In this study, the effect of several extraction methods, such as solid phase extraction (SPE), Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEChERS), and dispersive liquid-liquid micro-extraction (DLLME) was studied and the variable parameters that can arise throughout the extraction process were optimized to obtain the best results. A high-performance liquid chromatography with fluorescence detector (HPLC-FL) method was adapted and validated, including measurement uncertainty, for the analysis of melatonin in wines and to assess the efficiency of the extraction yield. After comparing the acquired results, the DLLME method was optimized. Extraction recoveries values ranging from 95 to 104% demonstrated that the approach may be successfully applied for the extraction and concentration (enrichment factor of almost eight) of melatonin in wine samples prior to HPLC-FL analysis. The first report of melatonin levels in Feteasca Neagra wines has been made. The data obtained for Cabernet Sauvignon revealed that the final levels of melatonin in the wines are dependent on the winemaking process.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28062768