Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis

Leptospirosis is an acute infection caused by pathogenic species of the genus Leptospira, which affects humans and animals in all world. In severe forms of the disease, kidneys, liver and lungs are the main affected organs, resulting in acute kidney injury, jaundice and pulmonary hemorrhage. Previou...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2020-01, Vol.62, p.e85-10
Main Authors: Iglezias, Silvia D'Andretta, Abreu, Patrícia Antonia Estima, Kanamura, Cristina, Magaldi, Antonio José, Seguro, Antonio Carlos, Brito, Thales De
Format: Article
Language:English
Subjects:
Acute Kidney Injury - microbiology
Acute Kidney Injury - pathology
Animals
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Female
Genes, Bacterial
Guinea Pigs
Humans
Kidney - pathology
Leptospira
Leptospira - genetics
Leptospira - metabolism
Leptospirosis
Leptospirosis - complications
Leptospirosis - metabolism
LipL32
Lipoproteins - genetics
Lipoproteins - metabolism
Lp25
Male
Middle Aged
Muscles - pathology
Myocardium - pathology
Original
Rhabdomyolysis
TROPICAL MEDICINE
Weil’s disease
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Summary:Leptospirosis is an acute infection caused by pathogenic species of the genus Leptospira, which affects humans and animals in all world. In severe forms of the disease, kidneys, liver and lungs are the main affected organs, resulting in acute kidney injury, jaundice and pulmonary hemorrhage. Previous post-mortem studies have shown that lesions are not limited to these organs. Cardiac and striated muscle injuries have already been reported, but the pathophysiology of cardiac and skeletal lesions in leptospirosis is not fully understood. It has been suggested that the tissue damage observed in leptospirosis could be directly mediated by leptospires or by their toxic cellular components. LipL32 and Lp25 are leptospira membrane proteins with unknown functions, that are present only in pathogenic strains of Leptospira spp. Both proteins induce skeletal muscle lesions similar to those observed when normal guinea pigs are inoculated with leptospires. Through immunohistochemistry, this study showed the presence of LipL32 and Lp25 proteins on muscle cell membranes and in the underlying cytoplasm of skeletal muscles, as well as focal lesions in cardiac tissues of fatal cases of leptospirosis. Altogether, these results reinforce that both proteins can be important factors in the pathogenesis of leptospirosis.
ISSN:1678-9946
0036-4665
1678-9946
DOI:10.1590/S1678-9946202062085