THE EFFECT OF THE SOMATOSTATIN ANALOGUE OCTREOTIDE ON EXPERIMENTAL INTESTINAL OBSTRUCTION IN RATS

• Published in: Acta cirúrgica brasileira 1998-10, Vol.13 (4), p.207-211
• Main Authors: Paran, Haim, Wider, Tzahi, Mayo, Ami, Neufeld, David, Susmalian, Sergio, Shwartz, Ivan, Freund, Uri
• Format: Article
• Language: English
• Subjects: Intestinal obstruction; Octreotide; Somatostatin
• ISSN: 0102-8650; 0102-8650; 1678-2674
• DOI: 10.1590/S0102-86501998000400001

Background: Somatostatin has an inhibitory effect on the endocrine and exocrine secretions of the gut. It may have a beneficial effect in the conservative treatment of intestinal obstruction. The aim of the present study is to investigate the effect of octreotide in mechanical intestinal obstruction in rats. Method: Intestinal obstruction was induced in rats by ligation of a segment of the distal ileum. Animals were treated with the somatostatin analogue octreotide (n=16), or saline (n=16). Eight rats were operated but their intestine was not ligated (n=8) serving as sham controls. Forty eight hours after the operation, the animals were operated upon again and blood samples from the femoral vein were tested for electrolytes, urea, glucose, lactic acid, amylase, ph and bicarbonate. Portal vein blood samples were also obtained and tested for lactic acid and amylase. Results: Intestinal obstruction resulted, after 48 hours, in severe dilatation of bowel loops. A significant increase in plasma levels of urea, amylase and lactic acid was observed. Plasma pH decreased. In blood samples from the portal vein, a significant increase in lactic acid was observed, indicating metabolic acidosis, probably secondary to bowel ischemia. Octreotide treatment, resulted in less acidosis, with concomitant lower urea and lactic acid levels in the plasma and especially in the portal vein. Conclusion: Octreotide treatment may have a beneficial effect in the conservative treatment of selected cases of intestinal obstruction. Objetivo: A somatostatina tem efeito inibidor nas secreτ⌡es end≤crina e ex≤crina do intestino. Poderß ter efeito benΘfico no tratamento conservador da obstruτπo intestinal. O objetivo do presente estudo Θ investigar o efeito do octreotide na obstruτπo mecΓnica do intestino delgado de ratos. MΘtodo: A obstruτπo intestinal foi induzida em ratos pela ligadura do segmento distal do ileum. Os animais foram tratados com somatostatina anßloga octreotide (n=16) ou com soluτπo salina (n=16). Oito ratos foram operados mas o intestino delgado nπo foi ligado (n=8) servindo como o grupo sham. Quarenta oito horas ap≤s a operaτπo os animais foram re-operados e submetidos a colheita de sangue da veia femoral a fim de verificar os eletr≤litos, urΘia, glicose, ßcido lßtico, amilase, pH e bicarbonato. Obteve-se tambΘm amostra de sangue da veia porta para verificar os nφveis de ßcido lßtico e amilase. Resultados: Ap≤s 48 horas de obstruτπo houve intensa dilataτπo das alτas intest