A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum

The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2014-07, Vol.109 (4), p.442-447
Main Authors: Freitas-Lidani, Kárita Cláudia, de Messias-Reason, Iara J, Ishikawa, Edna Aoba Y
Format: Article
Language:English
Subjects:
Animals
DNA Primers - genetics
DNA, Kinetoplast - genetics
DNA, Protozoan - genetics
Female
Genetic Markers
Insect Vectors - classification
Insect Vectors - parasitology
Leishmania (Leishmania) infantum
Leishmania infantum - genetics
Leishmania infantum - isolation & purification
Lutzomyia longipalpis
PARASITOLOGY
Polymerase Chain Reaction
Psychodidae - classification
Psychodidae - parasitology
Sensitivity and Specificity
TROPICAL MEDICINE
visceral leishmaniasis
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Summary:The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280 Lu. longipalpis female specimens were extracted from the whole insects. PCR primers for kinetoplast minicircle DNA (kDNA), the mini-exon gene and the small subunit ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the parasite was found with the kDNA primer in 8.6% of the cases, with the mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene primer in 5.3% of the cases. These data show the importance of polymerase chain reaction as a tool for investigating the molecular epidemiology of visceral leishmaniasis by estimating the risk of disease transmission in endemic areas, with the kDNA primer representing the most reliable marker for the parasite.
ISSN:1678-8060
0074-0276
1678-8060
DOI:10.1590/0074-0276130285