Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste

Recently, there has been a great deal of remarkable interest in finding bioactive compounds from nutritional foods to replace synthetic compounds. In particular, ortho-dihydroxyisoflavones and glycitein are of growing scientific interest owing to their attractive biological properties. In this study...

Full description

Saved in:
Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2014-12, Vol.4 (4), p.1093-1101
Main Author: Roh, Changhyun
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - metabolism
bioactive compound
biotransformation
Communication
Fermentation
Food Microbiology
Gas Chromatography-Mass Spectrometry
Isoflavones - metabolism
Isoflavones - pharmacokinetics
Magnetic Resonance Spectroscopy
Molecular Structure
screening
Soy Foods - microbiology
soybean paste
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253
cites cdi_FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253
container_end_page 1101
container_issue 4
container_start_page 1093
container_title Biomolecules (Basel, Switzerland)
container_volume 4
creator Roh, Changhyun
description Recently, there has been a great deal of remarkable interest in finding bioactive compounds from nutritional foods to replace synthetic compounds. In particular, ortho-dihydroxyisoflavones and glycitein are of growing scientific interest owing to their attractive biological properties. In this study, 7,8-ortho-dihydroxyisoflavone, 6,7-ortho-dihydroxyisoflavone, 3',4'-ortho-dihydroxyisoflavone and 7,4'-dihydroxy-6-methoxyisoflavone were characterized using microorganism screened from soybean Doenjang. Three ortho-dihydroxyisoflavones and glycitein were structurally elucidated by 1H-NMR and GC-MS analysis. Furthermore, bacterial strains from soybean Doenjang with the capacity of biotransformation were screened. The bacterial strain, identified as Bacillus subtilis Roh-1, was shown to convert daidzein into ortho-dihydroxyisoflavones and glycitein. Thus, this study has, for the first time, demonstrated that a bacterial strain had a substrate specificity for multiple modifications of the bioactive compounds.
doi_str_mv 10.3390/biom4041093
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_01ae47ba62bf4761b24bd59dd42760d1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_01ae47ba62bf4761b24bd59dd42760d1</doaj_id><sourcerecordid>1639486693</sourcerecordid><originalsourceid>FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253</originalsourceid><addsrcrecordid>eNqNks1rFDEYhwdRbKk9eZcBL4Ks5msmk4sgxWqh4kXBW8jnbpaZvGuSWZy_wn-5qduWrSdDICF5eN684dc0LzF6R6lA73WAiSGGkaBPmlNC8LAinP58erQ_ac5z3qI6hjoJfd6ckK7DlLPhtPnzNZgEOqixLUnF7CFNqgSILfi2ypUpYe9aA9MO5mhzq6JtdwnsbO4pSGUDKxs2i03wewkZ_Kj2EN0BXo-LCcWF2PoEUxtVmVOt5l2aXCzOthkW7VRsdyoX96J55tWY3fndetb8uPz0_eLL6vrb56uLj9cr06GhrBg33FAtlDHcWST4gJnvnTHVRCgjnGgthOZ26C2ng6HCOW8GZzzHuCMdPWuuDl4Lait3KUwqLRJUkH8PIK2lSiWY0UmElWNcq55oz3iPNWHadsLaWqZHFlfXh4NrN-vJWVPbqh0-kj6-iWEj17CXVSAwvxW8uRMk-DW7XOQUsnHjqKKDOUvcCyY4Hvr-P1AqWAUFrejrf9AtzCnWX60U6wijgqNKvT1QNQY5J-cf3o2RvI2YPIpYpV8dt_rA3geK3gCkGtF9</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1645243970</pqid></control><display><type>article</type><title>Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste</title><source>PubMed Central Free</source><source>ProQuest - Publicly Available Content Database</source><creator>Roh, Changhyun</creator><creatorcontrib>Roh, Changhyun</creatorcontrib><description>Recently, there has been a great deal of remarkable interest in finding bioactive compounds from nutritional foods to replace synthetic compounds. In particular, ortho-dihydroxyisoflavones and glycitein are of growing scientific interest owing to their attractive biological properties. In this study, 7,8-ortho-dihydroxyisoflavone, 6,7-ortho-dihydroxyisoflavone, 3',4'-ortho-dihydroxyisoflavone and 7,4'-dihydroxy-6-methoxyisoflavone were characterized using microorganism screened from soybean Doenjang. Three ortho-dihydroxyisoflavones and glycitein were structurally elucidated by 1H-NMR and GC-MS analysis. Furthermore, bacterial strains from soybean Doenjang with the capacity of biotransformation were screened. The bacterial strain, identified as Bacillus subtilis Roh-1, was shown to convert daidzein into ortho-dihydroxyisoflavones and glycitein. Thus, this study has, for the first time, demonstrated that a bacterial strain had a substrate specificity for multiple modifications of the bioactive compounds.</description><identifier>ISSN: 2218-273X</identifier><identifier>EISSN: 2218-273X</identifier><identifier>DOI: 10.3390/biom4041093</identifier><identifier>PMID: 25513748</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bacillus subtilis ; Bacillus subtilis - metabolism ; bioactive compound ; biotransformation ; Communication ; Fermentation ; Food Microbiology ; Gas Chromatography-Mass Spectrometry ; Isoflavones - metabolism ; Isoflavones - pharmacokinetics ; Magnetic Resonance Spectroscopy ; Molecular Structure ; screening ; Soy Foods - microbiology ; soybean paste</subject><ispartof>Biomolecules (Basel, Switzerland), 2014-12, Vol.4 (4), p.1093-1101</ispartof><rights>Copyright MDPI AG 2014</rights><rights>2014 by the authors; licensee MDPI, Basel, Switzerland. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253</citedby><cites>FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1645243970/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1645243970?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25513748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roh, Changhyun</creatorcontrib><title>Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste</title><title>Biomolecules (Basel, Switzerland)</title><addtitle>Biomolecules</addtitle><description>Recently, there has been a great deal of remarkable interest in finding bioactive compounds from nutritional foods to replace synthetic compounds. In particular, ortho-dihydroxyisoflavones and glycitein are of growing scientific interest owing to their attractive biological properties. In this study, 7,8-ortho-dihydroxyisoflavone, 6,7-ortho-dihydroxyisoflavone, 3',4'-ortho-dihydroxyisoflavone and 7,4'-dihydroxy-6-methoxyisoflavone were characterized using microorganism screened from soybean Doenjang. Three ortho-dihydroxyisoflavones and glycitein were structurally elucidated by 1H-NMR and GC-MS analysis. Furthermore, bacterial strains from soybean Doenjang with the capacity of biotransformation were screened. The bacterial strain, identified as Bacillus subtilis Roh-1, was shown to convert daidzein into ortho-dihydroxyisoflavones and glycitein. Thus, this study has, for the first time, demonstrated that a bacterial strain had a substrate specificity for multiple modifications of the bioactive compounds.</description><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - metabolism</subject><subject>bioactive compound</subject><subject>biotransformation</subject><subject>Communication</subject><subject>Fermentation</subject><subject>Food Microbiology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Isoflavones - metabolism</subject><subject>Isoflavones - pharmacokinetics</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Structure</subject><subject>screening</subject><subject>Soy Foods - microbiology</subject><subject>soybean paste</subject><issn>2218-273X</issn><issn>2218-273X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNks1rFDEYhwdRbKk9eZcBL4Ks5msmk4sgxWqh4kXBW8jnbpaZvGuSWZy_wn-5qduWrSdDICF5eN684dc0LzF6R6lA73WAiSGGkaBPmlNC8LAinP58erQ_ac5z3qI6hjoJfd6ckK7DlLPhtPnzNZgEOqixLUnF7CFNqgSILfi2ypUpYe9aA9MO5mhzq6JtdwnsbO4pSGUDKxs2i03wewkZ_Kj2EN0BXo-LCcWF2PoEUxtVmVOt5l2aXCzOthkW7VRsdyoX96J55tWY3fndetb8uPz0_eLL6vrb56uLj9cr06GhrBg33FAtlDHcWST4gJnvnTHVRCgjnGgthOZ26C2ng6HCOW8GZzzHuCMdPWuuDl4Lait3KUwqLRJUkH8PIK2lSiWY0UmElWNcq55oz3iPNWHadsLaWqZHFlfXh4NrN-vJWVPbqh0-kj6-iWEj17CXVSAwvxW8uRMk-DW7XOQUsnHjqKKDOUvcCyY4Hvr-P1AqWAUFrejrf9AtzCnWX60U6wijgqNKvT1QNQY5J-cf3o2RvI2YPIpYpV8dt_rA3geK3gCkGtF9</recordid><startdate>20141212</startdate><enddate>20141212</enddate><creator>Roh, Changhyun</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141212</creationdate><title>Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste</title><author>Roh, Changhyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - metabolism</topic><topic>bioactive compound</topic><topic>biotransformation</topic><topic>Communication</topic><topic>Fermentation</topic><topic>Food Microbiology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Isoflavones - metabolism</topic><topic>Isoflavones - pharmacokinetics</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Structure</topic><topic>screening</topic><topic>Soy Foods - microbiology</topic><topic>soybean paste</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roh, Changhyun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biomolecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roh, Changhyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste</atitle><jtitle>Biomolecules (Basel, Switzerland)</jtitle><addtitle>Biomolecules</addtitle><date>2014-12-12</date><risdate>2014</risdate><volume>4</volume><issue>4</issue><spage>1093</spage><epage>1101</epage><pages>1093-1101</pages><issn>2218-273X</issn><eissn>2218-273X</eissn><abstract>Recently, there has been a great deal of remarkable interest in finding bioactive compounds from nutritional foods to replace synthetic compounds. In particular, ortho-dihydroxyisoflavones and glycitein are of growing scientific interest owing to their attractive biological properties. In this study, 7,8-ortho-dihydroxyisoflavone, 6,7-ortho-dihydroxyisoflavone, 3',4'-ortho-dihydroxyisoflavone and 7,4'-dihydroxy-6-methoxyisoflavone were characterized using microorganism screened from soybean Doenjang. Three ortho-dihydroxyisoflavones and glycitein were structurally elucidated by 1H-NMR and GC-MS analysis. Furthermore, bacterial strains from soybean Doenjang with the capacity of biotransformation were screened. The bacterial strain, identified as Bacillus subtilis Roh-1, was shown to convert daidzein into ortho-dihydroxyisoflavones and glycitein. Thus, this study has, for the first time, demonstrated that a bacterial strain had a substrate specificity for multiple modifications of the bioactive compounds.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>25513748</pmid><doi>10.3390/biom4041093</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2218-273X
ispartof Biomolecules (Basel, Switzerland), 2014-12, Vol.4 (4), p.1093-1101
issn 2218-273X
2218-273X
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_01ae47ba62bf4761b24bd59dd42760d1
source PubMed Central Free; ProQuest - Publicly Available Content Database
subjects Bacillus subtilis
Bacillus subtilis - metabolism
bioactive compound
biotransformation
Communication
Fermentation
Food Microbiology
Gas Chromatography-Mass Spectrometry
Isoflavones - metabolism
Isoflavones - pharmacokinetics
Magnetic Resonance Spectroscopy
Molecular Structure
screening
Soy Foods - microbiology
soybean paste
title Microbial transformation of bioactive compounds and production of ortho-dihydroxyisoflavones and glycitein from natural fermented soybean paste
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T08%3A52%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microbial%20transformation%20of%20bioactive%20compounds%20and%20production%20of%20ortho-dihydroxyisoflavones%20and%20glycitein%20from%20natural%20fermented%20soybean%20paste&rft.jtitle=Biomolecules%20(Basel,%20Switzerland)&rft.au=Roh,%20Changhyun&rft.date=2014-12-12&rft.volume=4&rft.issue=4&rft.spage=1093&rft.epage=1101&rft.pages=1093-1101&rft.issn=2218-273X&rft.eissn=2218-273X&rft_id=info:doi/10.3390/biom4041093&rft_dat=%3Cproquest_doaj_%3E1639486693%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c508t-47c7c3b9acc7ed097814f6eccbea234272bb99b7d86d738c39eefc8ecf7115253%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1645243970&rft_id=info:pmid/25513748&rfr_iscdi=true