Identification and Characterization of Long Non-Coding RNAs: Implicating Insights into Their Regulatory Role in Kiwifruit Ripening and Softening during Low-Temperature Storage

Long non-coding RNAs (lncRNAs) are crucial players regulating many biological processes in plants. However, limited knowledge is available regarding their roles in kiwifruit ripening and softening. In this study, using lncRNA-seq technology, 591 differentially expressed (DE) lncRNAs (DELs) and 3107...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2023-02, Vol.12 (5), p.1070
Main Authors: Lai, Ruilian, Wu, Xiaopei, Feng, Xin, Gao, Minxia, Long, Yu, Wu, Rujian, Cheng, Chunzhen, Chen, Yiting
Format: Article
Language:English
Subjects:
Actinidia
Biological activity
Biosynthesis
Cell walls
Coding
Crops
Enrichment
fruit ripening and softening
Fruits
Genes
Genetic aspects
Growth
Kiwifruit
lncRNA-mRNA interaction
long non-coding RNA
Low temperature
low-temperature storage
Metabolism
Metabolites
Non-coding RNA
Pectinesterase
Physiology
Proteins
Ripening
Roles
Softening
Starch
Sucrose
Transcription factors
β-Amylase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Long non-coding RNAs (lncRNAs) are crucial players regulating many biological processes in plants. However, limited knowledge is available regarding their roles in kiwifruit ripening and softening. In this study, using lncRNA-seq technology, 591 differentially expressed (DE) lncRNAs (DELs) and 3107 DE genes (DEGs) were identified from kiwifruit stored at 4 °C for 1, 2, and 3 weeks in comparison with non-treated control fruits. Of note, 645 DEGs were predicted to be targets of DELs (DEGTLs), including some DE protein-coding genes (such as β-amylase and pectinesterase). DEGTL-based GO enrichment analysis revealed that these genes were significantly enriched in cell wall modification and pectinesterase activity in 1 W vs. CK and 3 W vs. CK, which might be closely related to the fruit softening during low-temperature storage. Moreover, KEGG enrichment analysis revealed that DEGTLs were significantly associated with starch and sucrose metabolism. Our study revealed that lncRNAs play critical regulatory roles in kiwifruit ripening and softening under low-temperature storage, mainly by mediating the expression of starch and sucrose metabolism and cell wall modification related genes.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12051070