Estimation of MBC: MIC Ratio of Herbal Extracts against Common Endodontic Pathogens

Herbal extracts have evoked interest owing to the small number of terpenoids and phenolic compounds, which impart antimicrobial, antioxidant, and anti-inflammatory properties. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (...

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Bibliographic Details
Published in:Journal of pharmacy & bioallied science 2024-04, Vol.16 (Suppl 2), p.S1414-S1416
Main Authors: Makade, Chetana S, Shenoi, Pratima R, Bhongade, Bhoomendra A, Shingane, Shrikant A, Ambulkar, Prajakta C, Shewale, Akhilesh M
Format: Article
Language:English
Subjects:
Actinomyces
Anti-inflammatory agents
Antibacterial agents
Antimicrobial activity
Antimicrobial agents
Bacteriostats
endodontic pathogens
Enterococcus faecalis
Essential oils
Fusobacterium nucleatum
herbal extracts
Lactobacillus
mbc
Medicine, Botanic
Medicine, Herbal
mic
mic: mbc
Minimum inhibitory concentration
Original
Pathogenic microorganisms
Pathogens
Peptostreptococcus
Phenolic compounds
Plant extracts
Staphylococcus aureus
Turbidity
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Summary:Herbal extracts have evoked interest owing to the small number of terpenoids and phenolic compounds, which impart antimicrobial, antioxidant, and anti-inflammatory properties. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of four herbal extracts (lemon grass oil, basil oil, peppermint oil, and Obicure tea extract) against endodontic pathogens along with the MIC: MBC/MFC ratio were evaluated. The antimicrobial activity by detecting the MIC of three essential oils and tea extract was evaluated against eight common endodontic pathogens by the broth dilution method, while MBC was detected by subculturing onto blood agar from the first -three to five tubes from the MIC dilution tubes (showing no turbidity), which were plated on blood agar. All herbal extracts proved to be effective antimicrobials against tested endodontic pathogens. Basil oil had a bacteriostatic effect on all the organisms ( < 0.05). Mint oil showed bacteriostatic activity on and ( > 0.05). Tea extract had a bacteriostatic effect ( > 0.05) against all tested microbes except Actinomyces, Lactobacilli, , and . Lemon grass oil had a bactericidal effect against all the organisms and a bacteriostatic effect against ( > 0.05). It can be concluded that basil oil showed a strong bactericidal effect on the test organisms. The MIC for the organisms ranged from 0.2 to 50 μg/ml.
ISSN:0976-4879
0975-7406
DOI:10.4103/jpbs.jpbs_735_23