Characterization of the wheat-tetraploid Thinopyrum elongatum 7E(7D) substitution line with Fusarium head blight resistance

• Published in: BMC plant biology 2024-10, Vol.24 (1), p.1006-1006, Article 1006
• Main Authors: Wu, Dandan, Wang, Fei, Chen, Linfeng, Mao, Yuanwen, Li, Yinghui, Zhu, Wei, Xu, Lili, Zhang, Yazhou, Wang, Yi, Zeng, Jian, Cheng, Yiran, Sha, Lina, Fan, Xing, Zhang, Haiqin, Zhou, Yonghong, Kang, Houyang
• Format: Article
• Language: English
• Subjects: abiotic stress; alleles; Blight; Chromosome 7; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Cloning; Common wheat substitution line; Control; Crop production; Cultivars; Disease resistance; Disease Resistance - genetics; Diseases and pests; dose response; durum wheat; Elongation; Environmental aspects; Fhb7 gene resistance; Fusarium; Fusarium - physiology; Fusarium graminearum; Fusarium head blight; Gene mapping; Genes; Genetic aspects; Genetic resources; Genomes; Germplasm; greenhouses; Growth; High resistance; Homology; Hybridization; Identification and classification; Marker-assisted selection; Plant Breeding; Plant Diseases - genetics; Plant Diseases - microbiology; Plant immunology; Poaceae - genetics; Poaceae - microbiology; Population genetics; Populations; Proteins; Seeds; Substitutes; substitution lines; Tetraploid Thinopyrum elongatum; Tetraploidy; Thinopyrum elongatum; Triticum - genetics; Triticum - microbiology; Triticum aestivum; Wheat
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05703-3

Fusarium head blight (FHB), a devastating disease of wheat production, is predominantly elicited by Fusarium graminearum (Fg). The tetraploid Thinopyrum elongatum is a tertiary gene resource of common wheat that possesses high affinity and displays high resistance traits against multiple biotic and abiotic stress. We aim to employ and utilize the novel FHB resistance resources from the wild germplasm of common wheat for breeding. Durum wheat-tetraploid Th. elongatum amphiploid 8801 was hybridized with common wheat cultivars SM482 and SM51, and the F generation was generated. We conducted cytogenetically in situ hybridization (ISH) technologies to select and confirm a genetically stable 7E(7D) substitution line K17-1069-5, which showed FHB expansion resistance in both field and greenhouse infection experiments and displayed no significant disadvantage in agronomic traits compared to their common wheat parents in the field. The F segregation populations (K17-1069-5 × SM830) showed that the 7E chromosome conferred dominant FHB resistance with dosage effect. We developed 19 SSR molecular markers specific to chromosome 7E, which could be conducted for genetic mapping and large breeding populations marker-assisted selection (MAS) during selection procedures in the future. We isolated a novel Fhb7 allele from the tetraploid Th. elongatum chromosome 7E (Chr7E) using homology-based cloning, which was designated as TTE7E-Fhb7. In summary, our study developed a novel wheat-tetraploid Thinopyrum elongatum 7E(7D) K17-1069-5 substitution line which contains stable FHB resistance.