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Evaluation of antigenicity and nutritional properties of enzymatically hydrolyzed cow milk
While enzymatic hydrolysis is an effective method for lowering the antigenicity of cow milk (CM), research regarding the antigenicity and nutritional traits of CM hydrolysate is limited. Here, we evaluated the protein content, amino acid composition, sensory traits, color, flow behavior, and antigen...
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Published in: | Scientific reports 2021-09, Vol.11 (1), p.18623-18623, Article 18623 |
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description | While enzymatic hydrolysis is an effective method for lowering the antigenicity of cow milk (CM), research regarding the antigenicity and nutritional traits of CM hydrolysate is limited. Here, we evaluated the protein content, amino acid composition, sensory traits, color, flow behavior, and antigenicity of CM following enzymatic hydrolysis. The results showed that enzymatic hydrolysis increased the degree of hydrolysis, destroyed allergenic proteins, including casein, β-lactoglobulin, and ɑ-lactalbumin, and significantly increased the content of free amino acids and nutritional quality. In particular, the antigenicity of CM was significantly reduced from 44.05 to 86.55% (
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P
< 0.5). Simultaneously, the taste, color, and flow behavior of CM were altered, the sweetness and richness intensity decreased significantly (
P
< 0.5), and astringency and bitterness were produced. A slightly darker and more yellow color was observed in CM hydrolysate. In addition, apparent viscosity decreased and shear stress significantly increased with increasing shear rate intensity. The results will provide a solid theoretical foundation for the development of high-quality hypoallergenic dairy products.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-98136-z</identifier><identifier>PMID: 34545177</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647 ; 631/250 ; 639/166 ; 639/638 ; Allergens ; Allergies ; Amino acid composition ; Amino acids ; Amino Acids - analysis ; Animals ; Antigenicity ; Asthma ; Astringents ; Bitterness ; Casein ; Color ; Cow's milk ; Dairy products ; Enzymes ; Food ; Food quality ; Humanities and Social Sciences ; Hydrolysates ; Hydrolysis ; Laboratory animals ; Lactalbumin ; Milk ; Milk - chemistry ; Milk - metabolism ; Milk Proteins - analysis ; multidisciplinary ; Nutrient content ; Nutritive Value ; Polyclonal antibodies ; Protein composition ; Proteins ; Rheology ; Science ; Science (multidisciplinary) ; Shear stress ; Sweetness ; β-Lactoglobulin</subject><ispartof>Scientific reports, 2021-09, Vol.11 (1), p.18623-18623, Article 18623</ispartof><rights>The Author(s) 2021</rights><rights>2021. The Author(s).</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-b20a53851fbf0b8d119732f53320b0e71c28ad80d22194bfae683ca6b5fcb773</citedby><cites>FETCH-LOGICAL-c540t-b20a53851fbf0b8d119732f53320b0e71c28ad80d22194bfae683ca6b5fcb773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2574548964/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2574548964?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25744,27915,27916,37003,37004,44581,53782,53784,74887</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34545177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Xiaona</creatorcontrib><creatorcontrib>Qian, Guanlin</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Yang, Mei</creatorcontrib><creatorcontrib>Shi, Xinyang</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Wu, Junrui</creatorcontrib><creatorcontrib>Wang, Zongzhou</creatorcontrib><creatorcontrib>Zheng, Yan</creatorcontrib><creatorcontrib>Yue, Xiqing</creatorcontrib><title>Evaluation of antigenicity and nutritional properties of enzymatically hydrolyzed cow milk</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>While enzymatic hydrolysis is an effective method for lowering the antigenicity of cow milk (CM), research regarding the antigenicity and nutritional traits of CM hydrolysate is limited. Here, we evaluated the protein content, amino acid composition, sensory traits, color, flow behavior, and antigenicity of CM following enzymatic hydrolysis. The results showed that enzymatic hydrolysis increased the degree of hydrolysis, destroyed allergenic proteins, including casein, β-lactoglobulin, and ɑ-lactalbumin, and significantly increased the content of free amino acids and nutritional quality. In particular, the antigenicity of CM was significantly reduced from 44.05 to 86.55% (
P
< 0.5). Simultaneously, the taste, color, and flow behavior of CM were altered, the sweetness and richness intensity decreased significantly (
P
< 0.5), and astringency and bitterness were produced. A slightly darker and more yellow color was observed in CM hydrolysate. In addition, apparent viscosity decreased and shear stress significantly increased with increasing shear rate intensity. The results will provide a solid theoretical foundation for the development of high-quality hypoallergenic dairy products.</description><subject>631/1647</subject><subject>631/250</subject><subject>639/166</subject><subject>639/638</subject><subject>Allergens</subject><subject>Allergies</subject><subject>Amino acid composition</subject><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Antigenicity</subject><subject>Asthma</subject><subject>Astringents</subject><subject>Bitterness</subject><subject>Casein</subject><subject>Color</subject><subject>Cow's milk</subject><subject>Dairy products</subject><subject>Enzymes</subject><subject>Food</subject><subject>Food quality</subject><subject>Humanities and Social Sciences</subject><subject>Hydrolysates</subject><subject>Hydrolysis</subject><subject>Laboratory animals</subject><subject>Lactalbumin</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Milk - metabolism</subject><subject>Milk Proteins - analysis</subject><subject>multidisciplinary</subject><subject>Nutrient content</subject><subject>Nutritive Value</subject><subject>Polyclonal antibodies</subject><subject>Protein composition</subject><subject>Proteins</subject><subject>Rheology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Shear stress</subject><subject>Sweetness</subject><subject>β-Lactoglobulin</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk1v1DAQhiMEolXpH-CAInHhEvBnbF-QUFWgUiUuPXGxbMfeenHsxU6Ksr8e76aUlgO--GOeeWfGM03zGoL3EGD-oRBIBe8Agp3gEPfd_llzigChHcIIPX90PmnOS9mCuigSBIqXzQkmlFDI2Gnz_fJOhVlNPsU2uVbFyW9s9MZPS70MbZyn7A9WFdpdTjubJ2_LAbVxv4zV0agQlvZ2GXIKy94OrUm_2tGHH6-aF06FYs_v97Pm5vPlzcXX7vrbl6uLT9edoQRMnUZAUcwpdNoBzQcIBcPIUYwR0MAyaBBXAwcDQlAQ7ZTtOTaq19QZzRg-a65W2SGprdxlP6q8yKS8PD6kvJGqJm2ClQABx3WN02NKuIFqIBhj3ve9AMRRXbU-rlq7WY92MDZOWYUnok8t0d_KTbqTnFDEAK8C7-4Fcvo52zLJ0RdjQ1DRprlIRBkFDFABK_r2H3Sb5lz_-UjVBnHRk0qhlTI5lZKte0gGAnkYBLkOQq0NyuMgyH11evO4jAeXP22vAF6BUk1xY_Pf2P-R_Q2E4cBO</recordid><startdate>20210920</startdate><enddate>20210920</enddate><creator>Liang, Xiaona</creator><creator>Qian, Guanlin</creator><creator>Sun, Jing</creator><creator>Yang, Mei</creator><creator>Shi, Xinyang</creator><creator>Yang, Hui</creator><creator>Wu, Junrui</creator><creator>Wang, Zongzhou</creator><creator>Zheng, Yan</creator><creator>Yue, Xiqing</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210920</creationdate><title>Evaluation of antigenicity and nutritional properties of enzymatically hydrolyzed cow milk</title><author>Liang, Xiaona ; Qian, Guanlin ; Sun, Jing ; Yang, Mei ; Shi, Xinyang ; Yang, Hui ; Wu, Junrui ; Wang, Zongzhou ; Zheng, Yan ; Yue, Xiqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-b20a53851fbf0b8d119732f53320b0e71c28ad80d22194bfae683ca6b5fcb773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/1647</topic><topic>631/250</topic><topic>639/166</topic><topic>639/638</topic><topic>Allergens</topic><topic>Allergies</topic><topic>Amino acid composition</topic><topic>Amino acids</topic><topic>Amino Acids - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals (DOAJ)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Xiaona</au><au>Qian, Guanlin</au><au>Sun, Jing</au><au>Yang, Mei</au><au>Shi, Xinyang</au><au>Yang, Hui</au><au>Wu, Junrui</au><au>Wang, Zongzhou</au><au>Zheng, Yan</au><au>Yue, Xiqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of antigenicity and nutritional properties of enzymatically hydrolyzed cow milk</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2021-09-20</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><spage>18623</spage><epage>18623</epage><pages>18623-18623</pages><artnum>18623</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>While enzymatic hydrolysis is an effective method for lowering the antigenicity of cow milk (CM), research regarding the antigenicity and nutritional traits of CM hydrolysate is limited. Here, we evaluated the protein content, amino acid composition, sensory traits, color, flow behavior, and antigenicity of CM following enzymatic hydrolysis. The results showed that enzymatic hydrolysis increased the degree of hydrolysis, destroyed allergenic proteins, including casein, β-lactoglobulin, and ɑ-lactalbumin, and significantly increased the content of free amino acids and nutritional quality. In particular, the antigenicity of CM was significantly reduced from 44.05 to 86.55% (
P
< 0.5). Simultaneously, the taste, color, and flow behavior of CM were altered, the sweetness and richness intensity decreased significantly (
P
< 0.5), and astringency and bitterness were produced. A slightly darker and more yellow color was observed in CM hydrolysate. In addition, apparent viscosity decreased and shear stress significantly increased with increasing shear rate intensity. The results will provide a solid theoretical foundation for the development of high-quality hypoallergenic dairy products.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>34545177</pmid><doi>10.1038/s41598-021-98136-z</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 631/1647 631/250 639/166 639/638 Allergens Allergies Amino acid composition Amino acids Amino Acids - analysis Animals Antigenicity Asthma Astringents Bitterness Casein Color Cow's milk Dairy products Enzymes Food Food quality Humanities and Social Sciences Hydrolysates Hydrolysis Laboratory animals Lactalbumin Milk Milk - chemistry Milk - metabolism Milk Proteins - analysis multidisciplinary Nutrient content Nutritive Value Polyclonal antibodies Protein composition Proteins Rheology Science Science (multidisciplinary) Shear stress Sweetness β-Lactoglobulin |
title | Evaluation of antigenicity and nutritional properties of enzymatically hydrolyzed cow milk |
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