Prognostic Significance of TWIST1 , CD24 , CD44 , and ALDH1 Transcript Quantification in EpCAM-Positive Circulating Tumor Cells from Early Stage Breast Cancer Patients

(1) Background: The aim of the study was to evaluate the prognostic significance of EMT-associated ( and stem-cell (SC) transcript ( , , ) quantification in EpCAM+ circulating tumor cells (CTCs) of early breast cancer patients. (2) Methods: 100 early stage breast cancer patients and 19 healthy donor...

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Published in:Cells (Basel, Switzerland) Switzerland), 2019-06, Vol.8 (7), p.652
Main Authors: Strati, Areti, Nikolaou, Michail, Georgoulias, Vassilis, Lianidou, Evi S
Format: Article
Language:English
Subjects:
Aldehyde Dehydrogenase 1 - metabolism
Biomarkers, Tumor - metabolism
Blood & organ donations
Breast cancer
Breast Neoplasms - mortality
Breast Neoplasms - pathology
Cancer therapies
CD24 Antigen - metabolism
CD44 antigen
Chemotherapy
circulating tumor cells
Disease-Free Survival
early breast cancer
Epithelial Cell Adhesion Molecule - metabolism
epithelial–mesenchymal transition
Female
Follow-Up Studies
Humans
Hyaluronan Receptors - metabolism
Hybridization
Kaplan-Meier Estimate
liquid biopsy
Lymph nodes
Medical prognosis
Metastasis
Middle Aged
Multivariate analysis
Nuclear Proteins - metabolism
Prognosis
Statistical analysis
Stem cells
Transcription
Tumor cells
Twist-Related Protein 1 - metabolism
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Summary:(1) Background: The aim of the study was to evaluate the prognostic significance of EMT-associated ( and stem-cell (SC) transcript ( , , ) quantification in EpCAM+ circulating tumor cells (CTCs) of early breast cancer patients. (2) Methods: 100 early stage breast cancer patients and 19 healthy donors were enrolled in the study. , , and transcripts of EpCAM cells were quantified using a novel highly sensitive and specific quadraplex RT-qPCR, while transcripts were quantified by single RT-qPCR. All patients were followed up for more than 5 years. (3) Results: A significant positive correlation between overexpression of and profile was found. Kaplan-Meier analysis revealed that the ER/PR-negative (HR-) patients and those patients with more than 3 positive lymph nodes that overexpressed in EpCAM cells had a significant lower DFI (log rank test; < 0.001, < 0.001) and OS (log rank test; = 0.006, < 0.001). Univariate and multivariate analysis also revealed the prognostic value of overexpression and and profile for both DFI and OS. (4) Conclusions: Detection of overexpression and stem-cell ( ) transcripts in EpCAM CTCs provides prognostic information in early stage breast cancer patients.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8070652