Chiral Liquid Crystal Microdroplets for Sensing Phospholipid Amphiphiles

Designing simple, sensitive, fast, and inexpensive readout devices to detect biological molecules and biomarkers is crucial for early diagnosis and treatments. Here, we have studied the interaction of the chiral liquid crystal (CLC) and biomolecules at the liquid crystal (LC)-droplet interface. CLC...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-05, Vol.12 (5), p.313
Main Authors: Norouzi, Sepideh, Martinez Gonzalez, Jose A, Sadati, Monirosadat
Format: Article
Language:English
Subjects:
Acids
Antimicrobial agents
Aqueous solutions
Bacteria
Biomarkers
Biomolecules
biosensing
chiral liquid crystal
Chirality
curved confinement
Design
Droplets
Glucose
Gram-positive bacteria
Image analysis
Image processing
Interfaces
Lecithin
Light microscopy
Lipids
Liquid crystals
Liquid Crystals - chemistry
Microfluidic devices
Microscopy
Optical microscopy
Phosphatidylcholine
Phosphatidylcholines
Phospholipids
Polyvinyl alcohol
Reconfiguration
Spectrometry
Spectroscopy
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Summary:Designing simple, sensitive, fast, and inexpensive readout devices to detect biological molecules and biomarkers is crucial for early diagnosis and treatments. Here, we have studied the interaction of the chiral liquid crystal (CLC) and biomolecules at the liquid crystal (LC)-droplet interface. CLC droplets with high and low chirality were prepared using a microfluidic device. We explored the reconfiguration of the CLC molecules confined in droplets in the presence of 1,2-diauroyl-sn-glycero3-phosphatidylcholine (DLPC) phospholipid. Cross-polarized optical microscopy and spectrometry techniques were employed to monitor the effect of droplet size and DLPC concentration on the structural reorganization of the CLC molecules. Our results showed that in the presence of DLPC, the chiral LC droplets transition from planar to homeotropic ordering through a multistage molecular reorientation. However, this reconfiguration process in the low-chirality droplets happened three times faster than in high-chirality ones. Applying spectrometry and image analysis, we found that the change in the chiral droplets' Bragg reflection can be correlated with the CLC-DLPC interactions.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12050313