Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis

Krüppel-like transcription factors (Klfs), which are characterized by the three conserved C-terminal zinc fingers, are involved in various biological processes, such as haematopoiesis and angiogenesis. However, how the Klf family of transcription factors cooperate in organogenesis remains elusive. D...

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Published in:Scientific reports 2023-08, Vol.13 (1), p.12279-12279, Article 12279
Main Authors: Suzuki, Hiroaki, Ogawa, Tomotaka, Fujita, Shigeyoshi, Sone, Ryota, Kawahara, Atsuo
Format: Article
Language:English
Subjects:
631/136/142
631/136/232/1473
Angiogenesis
c-Myb protein
CRISPR
Danio rerio
Embryogenesis
Embryonic growth stage
Erythroid cells
Erythropoiesis
Genome editing
Genomes
Hemopoiesis
Humanities and Social Sciences
Krueppel-like factor
multidisciplinary
Mutants
Organogenesis
Science
Science (multidisciplinary)
Transcription factors
Zinc finger proteins
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Summary:Krüppel-like transcription factors (Klfs), which are characterized by the three conserved C-terminal zinc fingers, are involved in various biological processes, such as haematopoiesis and angiogenesis. However, how the Klf family of transcription factors cooperate in organogenesis remains elusive. During zebrafish embryogenesis, both klf1 and klf17 are expressed in the intermediate cell mass (ICM), where primitive erythroid cells are produced. Using CRISPR–Cas9 genome editing technology, we established klf1 - klf17 double mutant zebrafish to investigate the functionally interactive roles of the klf1 and klf17 genes. The klf1 - klf17 mutant exhibited a diminished number of circulating primitive erythroid cells at 2 days postfertilization (dpf), while klf1 or klf17 single mutants and wild-type embryos produced comparable numbers of primitive erythroid cells. Circulating erythroid cells from the klf1 - klf17 mutant possessed larger nuclei at 2 dpf than wild-type cells, suggesting the impairment of primitive erythroid cell maturation. The expression of the erythroid cell maturation markers band3 and mitoferrin , but not the haematopoietic progenitor markers c-myb and scl , was decreased in the klf1 - klf17 mutant at 1 dpf. Thus, these results illustrate the cooperative function of klf1 and klf17 in the maturation processes of zebrafish primitive erythroid cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-39196-1