SNORA72 Activates the Notch1/c-Myc Pathway to Promote Stemness Transformation of Ovarian Cancer Cells

• Published in: Frontiers in cell and developmental biology 2020-11, Vol.8, p.583087-583087
• Main Authors: Zhang, Liwen, Ma, Rong, Gao, Mengcong, Zhao, Yanyun, Lv, Xuemei, Zhu, Wenjing, Han, Li, Su, Panpan, Fan, Yue, Yan, Yuanyuan, Zhao, Lin, Ma, Heyao, Wei, Minjie, He, Miao
• Format: Article
• Language: English
• Subjects: c-Myc; Cell and Developmental Biology; Notch1; ovarian cancer stem cells (OCSCs); SNORA72; stemness
• ISSN: 2296-634X; 2296-634X
• DOI: 10.3389/fcell.2020.583087

Cancer stem cells (CSCs) are responsible for the migration and recurrence of cancer progression. Small nucleolar RNAs (snoRNAs) play important roles in tumor development. However, how snoRNAs contribute to the regulation of the stemness of ovarian CSCs (OCSCs) remains unclear. In the present study, we found that SNORA72 was significantly upregulated in OVCAR-3 spheroids (OS) and CAOV-3 spheroids (CS) with the OCSC characteristics attained by serum-free culture in a suspension of OVCAR-3 (OV) and CAOV-3 (CA) cells. The overexpression of SNORA72 increased self-renewal abilities and migration abilities in OV and CA cells and upregulated the expressions of the stemness markers Nanog, Oct4, and CD133. In addition, the ectopic expression of SNORA72 can elevate the messenger RNA (mRNA) and protein expression levels of Notch1 and c-Myc in parental cells. The opposite results were observed in SNORA72-silenced OCSCs. Moreover, we found that Notch1 knockdown inversed the migration abilities and self-renewal abilities raised by overexpressing SNORA72. In summary, stemness transformation of ovarian cancer cells can be activated by SNORA72 through the Notch1/c-Myc pathway. This study introduces a novel therapeutic strategy for improving the treatment efficiency of ovarian cancer.