Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

The use of plasticizers to improve the quality of plastics widely used for household purposes inevitably leads to an increase in their pollution of food and environmental objects. Diisobutyl phthalate (DiBP) is one of the ortho-substituted phthalic acid esters that negatively affect human health and...

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Bibliographic Details
Published in:Chemosensors 2023-07, Vol.11 (7), p.393
Main Authors: Berlina, Anna N, Ragozina, Maria Y, Gusev, Daniil I, Zherdev, Anatoly V, Dzantiev, Boris B
Format: Article
Language:English
Subjects:
Antibodies
Aquatic organisms
Chemical properties
Chemiluminescence
Chromatography
contaminant
diisobutyl phthalate
ELISA
Enzyme immunoassay
Enzyme-linked immunosorbent assay
Enzymes
Esters
Food
Food packaging
Food products
Hexanes
Horseradish peroxidase
Hydrogen peroxide
Identification and classification
Immunoassay
Methanol
Methods
PAEs
Peroxidase
Phthalates
Phthalic acid
plastic
Polyclonal antibodies
Recovery
Seafood
Substrates
Viral antibodies
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Summary:The use of plasticizers to improve the quality of plastics widely used for household purposes inevitably leads to an increase in their pollution of food and environmental objects. Diisobutyl phthalate (DiBP) is one of the ortho-substituted phthalic acid esters that negatively affect human health and ecosystems. This work is directed to the development of a chemiluminescent enzyme immunoassay (CL-ELISA) for the determination of diisobutyl phthalate in water and food. Luminol, which is oxidized with hydrogen peroxide in the presence of p-iodophenol as an enhancer, was chosen as the substrate for horseradish peroxidase used as a label in the analysis. For this development, rabbit anti-DiBP polyclonal antibodies were generated and tested with the synthesized hapten–protein conjugate. The developed chemiluminescent ELISA has a detection limit of 1.8 ng/mL; the operating range was 5.0–170.8 ng/mL at a content of 10% methanol in the assay medium. The assay was successfully applied to detect diisobutyl phthalate in lettuce leaves, seafood, and water. When using extraction with methanol and hexane, the recovery of DiBP in samples varies in the range of 76.9–134.2%; for assays in natural waters, the recovery rates are from 79.5 to 113.4%.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors11070393