Chemical Characterization and Several Bioactivities of Cladanthus mixtus from Morocco

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-04, Vol.28 (7), p.3196
• Main Authors: El Mihyaoui, Amina, Erbiai, El Hadi, Charfi, Saoulajan, Pinto, Eugénia, Castillo, María Emilia Candela, Hernández-Ruiz, Josefa, Cano, Antonio, Badoc, Alain, Lamarti, Ahmed, Esteves da Silva, Joaquim C G, Arnao, Marino B
• Format: Article
• Language: English
• Subjects: Amino acids; antibacterial activity; Antifungal activity; Antimicrobial activity; antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Aspergillus fumigatus; Biochemistry; Biological activity; Cancer; Candida albicans; Carbohydrates; Chemical composition; Chemical compounds; Cladanthus mixtus; Dilution; E coli; Environmental Sciences; Escherichia coli; Fatty acids; Flavonoids; Flavonoids - analysis; Flavonoids - pharmacology; Flowers; Flowers & plants; Fungicides; Gallic acid; Gas chromatography; Herbal medicine; Leaves; Mass spectrometry; Mass spectroscopy; Methanol - pharmacology; Minimum inhibitory concentration; Morocco; Organic acids; Pathogens; Phenolic compounds; Phenols; Phenols - analysis; Phenols - pharmacology; Plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Quercetin; Staphylococcus aureus; Sucrose; Sulfonic acid; Trichophyton rubrum; Vitamin E
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28073196

The purpose of this work was to investigate, for the first time to our knowledge, the chemical composition and bioactivity of methanolic extracts (roots, stems, leaves, and flowers) from (L.) Chevall. that grows wild in northern Morocco (the Tangier-Tetouan-Al Hoceima region). The phenolic and flavonoid contents were determined by spectrophotometer methods, and the composition of derivatized methanolic extracts from using - -bis(trimethylsilyl) trifluoroacetamide (BSTFA) was analyzed by gas chromatography-mass spectrometry (GC-MS). The antioxidant activity was carried out by applying the 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and DPPH (2,2-diphenyl-1-picrylhydrazyl) tests. The micro-dilution technique was chosen to investigate the antimicrobial activity of methanolic extracts against two bacterial strains and three fungal species. The results showed that the values of total phenolic and flavonoid contents were found to be higher in flower extracts (30.55 ± 0.85 mg of gallic acid equivalents (GAE)/g of dried weight (DW) and 26.00 ±1.34 mg of quercetin equivalents (QE)/g DW, respectively). Other groups of chemical compounds were revealed by GC-MS, such as carbohydrates (27.25-64.87%), fatty acids (1.58-9.08%), organic acids (11.81-18.82%), and amino acids (1.26-7.10%). Root and flower methanolic extracts showed the highest antioxidant activity using ABTS (39.49 mg of Trolox equivalents (TE)/g DW) and DPPH (36.23 mg TE/g DW), respectively. A positive correlation between antioxidant activity and polyphenol and flavonoid amounts was found. Antibacterial tests showed that the best activity was presented by the leaf extract against (minimum inhibitory concentration (MIC) = minimum bactericidal concentration (MBC) = 20 mg/mL) and (MIC of 30 mg/mL and MBC of 35 mg/mL). was more sensitive to the extracts compared to . All extracts showed antifungal activity against , with the best efficacy reported by the flower and leaf extracts (MIC = 1.25 mg/mL and minimum fungicidal concentration (MFC) = 2.5 mg/mL). In general, extracts of appeared less effective against and .