Evaluation of the Skin Sensitization Potential of Carbon Nanotubes Using Alternative In Vitro and In Vivo Assays

Carbon nanotubes (CNTs) are one of the major types of nanomaterials that have various industrial and biomedical applications. However, there is a risk of accidental exposure to CNTs in individuals involved in their large-scale production and in individuals who use products containing CNTs. This stud...

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Bibliographic Details
Published in:Toxics (Basel) 2020-12, Vol.8 (4), p.122
Main Authors: Kim, Sung-Hyun, Lee, Dong Han, Lee, Jin Hee, Yang, Jun-Young, Shin, Hyo-Sook, Lee, JeongPyo, Jung, Kikyung, Jeong, Jayoung, Oh, Jae-Ho, Lee, Jong Kwon
Format: Article
Language:English
Subjects:
alternative to animal testing
Assaying
Biomedical materials
Carbon
CNT
Evaluation
In vitro methods and tests
In vivo methods and tests
KeratinoSens
LLNA
Lymphatic system
Morphology
Multi wall carbon nanotubes
nanomaterial
Nanomaterials
Nanotechnology
Nanotubes
Peptides
Physiochemistry
Single wall carbon nanotubes
Skin
skin sensitization
Test methods
Zeta potential
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Summary:Carbon nanotubes (CNTs) are one of the major types of nanomaterials that have various industrial and biomedical applications. However, there is a risk of accidental exposure to CNTs in individuals involved in their large-scale production and in individuals who use products containing CNTs. This study aimed to evaluate the skin sensitization induced by CNTs using two alternative tests. We selected single-wall carbon nanotubes and multi-walled carbon nanotubes for this study. First, the physiochemical properties of the CNTs were measured, including the morphology, size, and zeta potential, under various conditions. Thereafter, we assessed the sensitization potential of the CNTs using the ARE-Nrf2 Luciferase KeratinoSens™ assay, an in vitro alternative test method. In addition, the CNTs were evaluated for their skin sensitization potential using the LLNA: BrdU-FCM in vivo alternative test method. In this study, we report for the first time the sensitization results of CNTs using the KeratinoSens™ and LLNA: BrdU-FCM test methods in this study. This study found that both CNTs do not induce skin sensitization. These results suggest that the KeratinoSens™ and LLNA: BrdU-FCM assay may be useful as alternative assays for evaluating the potential of some nanomaterials that can induce skin sensitization.
ISSN:2305-6304
2305-6304
DOI:10.3390/toxics8040122