Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform, CellSaic, in a Rat Congenital Cleft-Jaw Model

Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™ to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic containing r...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (17), p.9478
Main Authors: Lyu, Jinzhao, Hashimoto, Yoshiya, Honda, Yoshitomo, Matsumoto, Naoyuki
Format: Article
Language:English
Subjects:
Biomedical materials
Bone growth
Bone marrow
Bone marrow transplantation
Calcium
Cbfa-1 protein
Cell differentiation
Cell proliferation
Cellnest
CellSaic
Collagen
Congenital defects
Dental pulp
Differentiation
Efficiency
Gene expression
Jaw
Mesenchymal stem cells
Morphology
Nutrition
Osteoblastogenesis
rBMSCs
rDPSCs
Regeneration
Regeneration (physiology)
Repair
Scaffolds
Stem cell transplantation
Stem cells
Tissue engineering
Transplantation
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Summary:Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™ to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic containing rat dental pulp stem cells (rDPSCs) was better than that containing rat bone marrow stem cells (rBMSCs). rDPSC-CellSaic or rBMSC-CellSaic, cultured with or without osteogenic induction medium, formed the experimental and control groups, respectively. Osteoblast differentiation was evaluated in vitro and transplanted into a rat model with a congenital jaw fracture. Specimens were collected and evaluated by microradiology and histological analysis. In the experimental group, the amount of calcium deposits, expression levels of bone-related genes (RUNX2, ALP, BSP, and COL1), and volume of mineralized tissue, were significantly higher than those in the control group (p < 0.05). Both differentiated and undifferentiated rDPSC-CellSaic and only the differentiated rBMSC-CellSaic could induce the formation of new bone tissue. Overall, rBMSC-CellSaic and rDPSC-CellSaic made with Cellnest™ as a scaffold, provide excellent support for promoting bone regeneration in rat mandibular congenital defects. Additionally, rDPSC-CellSaic seems a better source for craniofacial bone defect repair than rBMSC-CellSaic, suggesting the possibility of using DPSCs in bone tissue regenerative therapy.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22179478