Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans

Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR)...

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Bibliographic Details
Published in:Parasite (Paris) 2022, Vol.29, p.30-30
Main Authors: Šloufová, Martina, Lhotská, Zuzana, Jirků, Milan, Petrželková, Klára J, Stensvold, C Rune, Cinek, Ondřej, Pomajbíková, Kateřina Jirků
Format: Article
Language:English
Subjects:
Blastocystis
Blastocystis - genetics
Blastocystis Infections - diagnosis
Blastocystis Infections - epidemiology
Colonization
conventional-pcr
Deoxyribonucleic acid
Differentiation
DNA
Epidemiology
Feces
Humans
Intestine
Next-generation sequencing
ngs
Pathology, Molecular
Polymerase chain reaction
qpcr
quantification
Real-Time Polymerase Chain Reaction
Sensitivity
Surveillance
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Summary:Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies.
ISSN:1776-1042
1252-607X
1776-1042
DOI:10.1051/parasite/2022029