Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars

Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sen...

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Bibliographic Details
Published in:Biosensors (Basel) 2019-06, Vol.9 (3), p.83
Main Authors: Phiri, Masauso Moses, Mulder, Danielle Wingrove, Vorster, Barend Christiaan
Format: Article
Language:English
Subjects:
Biocatalysis
biocatalytic shape-altering
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Blood Glucose
Cell culture
Clustering
colorimetric detection
Colorimetry
Dilution
Enzyme Stability
Enzymes
Enzymes, Immobilized
Glucose
glucose biosensor
Glucose monitoring
Glucose Oxidase
Gold
gold nanostars
Laboratory equipment
localized surface plasmon resonance
Metal Nanoparticles
Methods
Nanoparticles
Nanotechnology
NMR
Nuclear magnetic resonance
Optical communication
Sensitivity
Silver
Spectrophotometry
Spectrum Analysis
Surface Plasmon Resonance - methods
Technology transfer
Transducers
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Summary:Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios9030083