Loading…
Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars
Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sen...
Saved in:
| Published in: | Biosensors (Basel) 2019-06, Vol.9 (3), p.83 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63 |
| container_end_page | |
| container_issue | 3 |
| container_start_page | 83 |
| container_title | Biosensors (Basel) |
| container_volume | 9 |
| creator | Phiri, Masauso Moses Mulder, Danielle Wingrove Vorster, Barend Christiaan |
| description | Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices. |
| doi_str_mv | 10.3390/bios9030083 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_02d8c5d54837426b9d2c7360fd5d7501</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_02d8c5d54837426b9d2c7360fd5d7501</doaj_id><sourcerecordid>2547475507</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63</originalsourceid><addsrcrecordid>eNpVkU1v1DAQhi0EolXpiTuKxBGlOP6a-ILUFloqVYBUuGI59mTrVTZebAep_x63W6qtL_6Ydx7PzEvI246ecK7pxyHErCmntOcvyCGjoFvFQbzcOx-Q45zXtC4QoDm8Jge8Y6rTQh-S3z8mmzdxDq75jAVdCXFu4thcTouLGZswNzeYlk1zZjP6pgbPQnS22Omu1JybW7vF9nQqmMK8ekiMk2--2TnmYlN-Q16Ndsp4_LgfkV8XX36ef22vv19enZ9et06ALC3DAcDz-46skzAgF-Co7KRDq0ZJe-hcbzUKZjlgvY2qU8OAgxZMS6_4EbnacX20a7NNYWPTnYk2mIeHmFbGplrwhIYy3zvppejrbJgatGcOuKKjlx4k7Srr0461XYYNeodzSXZ6Bn0emcOtWcW_RkEvOMgKeP8ISPHPgrmYdVzSXPs3TIpqgpQUqurDTuVSzDnh-PRDR839JMyet1X9br-oJ-1_J_k_NTKfDw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2547475507</pqid></control><display><type>article</type><title>Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars</title><source>Open Access: PubMed Central</source><source>Publicly Available Content (ProQuest)</source><creator>Phiri, Masauso Moses ; Mulder, Danielle Wingrove ; Vorster, Barend Christiaan</creator><creatorcontrib>Phiri, Masauso Moses ; Mulder, Danielle Wingrove ; Vorster, Barend Christiaan</creatorcontrib><description>Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices.</description><identifier>ISSN: 2079-6374</identifier><identifier>EISSN: 2079-6374</identifier><identifier>DOI: 10.3390/bios9030083</identifier><identifier>PMID: 31261949</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biocatalysis ; biocatalytic shape-altering ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Blood Glucose ; Cell culture ; Clustering ; colorimetric detection ; Colorimetry ; Dilution ; Enzyme Stability ; Enzymes ; Enzymes, Immobilized ; Glucose ; glucose biosensor ; Glucose monitoring ; Glucose Oxidase ; Gold ; gold nanostars ; Laboratory equipment ; localized surface plasmon resonance ; Metal Nanoparticles ; Methods ; Nanoparticles ; Nanotechnology ; NMR ; Nuclear magnetic resonance ; Optical communication ; Sensitivity ; Silver ; Spectrophotometry ; Spectrum Analysis ; Surface Plasmon Resonance - methods ; Technology transfer ; Transducers</subject><ispartof>Biosensors (Basel), 2019-06, Vol.9 (3), p.83</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63</citedby><cites>FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63</cites><orcidid>0000-0001-7653-7988 ; 0000-0003-2371-288X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2547475507/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2547475507?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25732,27903,27904,36991,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31261949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phiri, Masauso Moses</creatorcontrib><creatorcontrib>Mulder, Danielle Wingrove</creatorcontrib><creatorcontrib>Vorster, Barend Christiaan</creatorcontrib><title>Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars</title><title>Biosensors (Basel)</title><addtitle>Biosensors (Basel)</addtitle><description>Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices.</description><subject>Biocatalysis</subject><subject>biocatalytic shape-altering</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Blood Glucose</subject><subject>Cell culture</subject><subject>Clustering</subject><subject>colorimetric detection</subject><subject>Colorimetry</subject><subject>Dilution</subject><subject>Enzyme Stability</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized</subject><subject>Glucose</subject><subject>glucose biosensor</subject><subject>Glucose monitoring</subject><subject>Glucose Oxidase</subject><subject>Gold</subject><subject>gold nanostars</subject><subject>Laboratory equipment</subject><subject>localized surface plasmon resonance</subject><subject>Metal Nanoparticles</subject><subject>Methods</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Optical communication</subject><subject>Sensitivity</subject><subject>Silver</subject><subject>Spectrophotometry</subject><subject>Spectrum Analysis</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Technology transfer</subject><subject>Transducers</subject><issn>2079-6374</issn><issn>2079-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpVkU1v1DAQhi0EolXpiTuKxBGlOP6a-ILUFloqVYBUuGI59mTrVTZebAep_x63W6qtL_6Ydx7PzEvI246ecK7pxyHErCmntOcvyCGjoFvFQbzcOx-Q45zXtC4QoDm8Jge8Y6rTQh-S3z8mmzdxDq75jAVdCXFu4thcTouLGZswNzeYlk1zZjP6pgbPQnS22Omu1JybW7vF9nQqmMK8ekiMk2--2TnmYlN-Q16Ndsp4_LgfkV8XX36ef22vv19enZ9et06ALC3DAcDz-46skzAgF-Co7KRDq0ZJe-hcbzUKZjlgvY2qU8OAgxZMS6_4EbnacX20a7NNYWPTnYk2mIeHmFbGplrwhIYy3zvppejrbJgatGcOuKKjlx4k7Srr0461XYYNeodzSXZ6Bn0emcOtWcW_RkEvOMgKeP8ISPHPgrmYdVzSXPs3TIpqgpQUqurDTuVSzDnh-PRDR839JMyet1X9br-oJ-1_J_k_NTKfDw</recordid><startdate>20190629</startdate><enddate>20190629</enddate><creator>Phiri, Masauso Moses</creator><creator>Mulder, Danielle Wingrove</creator><creator>Vorster, Barend Christiaan</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7653-7988</orcidid><orcidid>https://orcid.org/0000-0003-2371-288X</orcidid></search><sort><creationdate>20190629</creationdate><title>Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars</title><author>Phiri, Masauso Moses ; Mulder, Danielle Wingrove ; Vorster, Barend Christiaan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biocatalysis</topic><topic>biocatalytic shape-altering</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Blood Glucose</topic><topic>Cell culture</topic><topic>Clustering</topic><topic>colorimetric detection</topic><topic>Colorimetry</topic><topic>Dilution</topic><topic>Enzyme Stability</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized</topic><topic>Glucose</topic><topic>glucose biosensor</topic><topic>Glucose monitoring</topic><topic>Glucose Oxidase</topic><topic>Gold</topic><topic>gold nanostars</topic><topic>Laboratory equipment</topic><topic>localized surface plasmon resonance</topic><topic>Metal Nanoparticles</topic><topic>Methods</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Optical communication</topic><topic>Sensitivity</topic><topic>Silver</topic><topic>Spectrophotometry</topic><topic>Spectrum Analysis</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Technology transfer</topic><topic>Transducers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phiri, Masauso Moses</creatorcontrib><creatorcontrib>Mulder, Danielle Wingrove</creatorcontrib><creatorcontrib>Vorster, Barend Christiaan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biosensors (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phiri, Masauso Moses</au><au>Mulder, Danielle Wingrove</au><au>Vorster, Barend Christiaan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars</atitle><jtitle>Biosensors (Basel)</jtitle><addtitle>Biosensors (Basel)</addtitle><date>2019-06-29</date><risdate>2019</risdate><volume>9</volume><issue>3</issue><spage>83</spage><pages>83-</pages><issn>2079-6374</issn><eissn>2079-6374</eissn><abstract>Nanoparticles have been used as signal transducers for optical readouts in biosensors. Optical approaches are cost-effective with easy readout formats for clinical diagnosis. We present a glucose biosensor based on the biocatalytic shape-altering of gold nanostars via silver deposition. Improved sensitivity was observed due to the nanostars clustering after being functionalised with glucose oxidase (GOx). The biosensor quantified glucose in the serum samples with a 1:1000 dilution factor, and colorimetrically distinguished between the concentrations. The assay demonstrated good specificity and sensitivity. The fabricated glucose biosensor is a rapid kinetic assay using a basic entry level laboratory spectrophotometric microplate reader. Such a biosensor could be very useful in resource-constrained regions without state-of-the-art laboratory equipment. Furthermore, naked eye detection of glucose makes this a suitable biosensor for technology transfer to other point-of-care devices.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31261949</pmid><doi>10.3390/bios9030083</doi><orcidid>https://orcid.org/0000-0001-7653-7988</orcidid><orcidid>https://orcid.org/0000-0003-2371-288X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2079-6374 |
| ispartof | Biosensors (Basel), 2019-06, Vol.9 (3), p.83 |
| issn | 2079-6374 2079-6374 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_02d8c5d54837426b9d2c7360fd5d7501 |
| source | Open Access: PubMed Central; Publicly Available Content (ProQuest) |
| subjects | Biocatalysis biocatalytic shape-altering Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Blood Glucose Cell culture Clustering colorimetric detection Colorimetry Dilution Enzyme Stability Enzymes Enzymes, Immobilized Glucose glucose biosensor Glucose monitoring Glucose Oxidase Gold gold nanostars Laboratory equipment localized surface plasmon resonance Metal Nanoparticles Methods Nanoparticles Nanotechnology NMR Nuclear magnetic resonance Optical communication Sensitivity Silver Spectrophotometry Spectrum Analysis Surface Plasmon Resonance - methods Technology transfer Transducers |
| title | Plasmonic Detection of Glucose in Serum Based on Biocatalytic Shape-Altering of Gold Nanostars |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T01%3A41%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasmonic%20Detection%20of%20Glucose%20in%20Serum%20Based%20on%20Biocatalytic%20Shape-Altering%20of%20Gold%20Nanostars&rft.jtitle=Biosensors%20(Basel)&rft.au=Phiri,%20Masauso%20Moses&rft.date=2019-06-29&rft.volume=9&rft.issue=3&rft.spage=83&rft.pages=83-&rft.issn=2079-6374&rft.eissn=2079-6374&rft_id=info:doi/10.3390/bios9030083&rft_dat=%3Cproquest_doaj_%3E2547475507%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c475t-2eb77d33390ac57be347c0515cea6f50871c8a9e42a37e871f616bbeb94295d63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2547475507&rft_id=info:pmid/31261949&rfr_iscdi=true |