Point Mutation in Factor V Leiden G1691A and Factor II G20210A and Effect on Coagulation Profile and Frequency of Recurrent Spontaneous Abortions among Sudanese Women

• Published in: J. Islamic Int. Med. Coll. 2016-09, Vol.11 (3), p.108-112
• Main Author: Asaad Mohammed Ahmed Abd Allah Babker , Fath Elrahman Mahdi Hassan Gameel , Salaheldein Gumaa Elzaki , 4 5 6 Amanda G Elgoraish , Lienda Bashier Eltayeb , Hisham Ali Waggiallah
• Format: Article
• Language: English
• Subjects: factor v leiden g1691a, factor ii g20210a, rsa, sudanese pregnant women
• ISSN: 1815-4018; 2410-5422

Objective: To investigate the effect of point mutation in FV Leiden G1691A and FII G20210A gene on coagulation and recurrent spontaneous abortion (RSA) among Sudanese women. Study Design: This was retrospective case control study. Place and Duration of Study: The study was carried out from Aug 2012 to Dec 2014 at Omdurman Maternal Hospital, Sudan. Materials and Methods: The study included hundred pregnant females with a history of recurrent spontaneous abortion as (case group) and ninety five healthy reproductive Sudanese women as (control group). The data was collected with the help of structured questionnaire and direct interview to collect information. Identification of point mutation in factor V Leiden G1691A and factor II G20210A gene by polymerase chain reaction was performed; Coagulometer was used for the measurement of PT and APTT. Odds Ratio and the 95% confidence interval (95%CI) were calculated for the presence of mutation between cases and controls and analyzed by SPSS program, version 17.0. Results: The Heterozygous alleles G/A in factor V gene was 8.0% in all cases related with three, four and five times of recurrent abortion and 6% was found in control group. Heterozygous alleles of factor II G/A Prothrombin time (PT) and partial thromboplastin time (PTT) in women with Recusant Spontaneous Abortion (RSA) were not affected significantly (P=0. 93 and P=0.69). Conclusion: Based upon the results it is concluded that the point mutation in factor V Leiden G1691A and factor II G20210A might play a role in recurrent spontaneous abortion loss among Sudanese women. However these point mutations do not affect the coagulation profile.