A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes

Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcription...

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Bibliographic Details
Published in:eLife 2018-06, Vol.7
Main Authors: Teves, Sheila S, An, Luye, Bhargava-Shah, Aarohi, Xie, Liangqi, Darzacq, Xavier, Tjian, Robert
Format: Article
Language:English
Subjects:
Animals
Binding sites
Cell cycle
Cell Line
Chromatin
Chromosomes - chemistry
Chromosomes - metabolism
Chromosomes and Gene Expression
Diterpenes - pharmacology
DNA-directed RNA polymerase
Embryo cells
Epoxy Compounds - pharmacology
Flavonoids - pharmacology
Genomes
Mice
Mitosis
Mitosis - drug effects
Molecular Imaging
mouse embryonic stem cells
Mouse Embryonic Stem Cells - cytology
Mouse Embryonic Stem Cells - drug effects
Mouse Embryonic Stem Cells - metabolism
Phenanthrenes - pharmacology
Piperidines - pharmacology
Principal components analysis
Promoter Regions, Genetic
Protein Binding - drug effects
RNA Polymerase II - genetics
RNA Polymerase II - metabolism
single molecule live-cell imaging
Single-Cell Analysis
Stem cell transplantation
Stem cells
TATA-binding protein
TATA-Box Binding Protein - genetics
TATA-Box Binding Protein - metabolism
TBP
Transcription
transcription factors
Transcriptional Activation
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Summary:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.35621