Assessing the In Vitro Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model

Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic digestion conditions are still very scarce. To address this gap, th...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2023-08, Vol.13 (15), p.2237
Main Authors: Madalena, Daniel A, Araújo, João F, Ramos, Óscar L, Vicente, António A, Pinheiro, Ana C
Format: Article
Language:English
Subjects:
bioaccessibility
Calibration
Confocal microscopy
Curcumin
Digestion
dynamic gastric digestion
Enzymes
Ethanol
Food products
in vitro digestion
Lactoferrin
Light scattering
nanodelivery system
Nanoparticles
nanostructures
Photon correlation spectroscopy
Polydispersity
Proteins
Scanning microscopy
Size distribution
Spectroscopy
Stomach
Zeta potential
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Summary:Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic digestion conditions are still very scarce. To address this gap, this study aims to assess the dynamic gastric digestion of lactoferrin/curcumin nanoparticles in the realistic gastric model (RGM). For this purpose, the INFOGEST standard semi-dynamic digestion protocol was used. The nanosystems were characterized in terms of hydrodynamic size, size distribution, polydispersity index (PdI), and zeta potential using dynamic light scattering (DLS), before and during the digestion process. Confocal laser scanning microscopy (CLSM) was also used to examine particle aggregation. In addition, the release of curcumin was evaluated spectroscopically and the intrinsic fluorescence of lactoferrin was measured throughout the digestion process. The protein hydrolysis was also determined by UV-VIS-SWNIR spectroscopy to estimate, in real-time, the presence of free NH2 groups during gastric digestion. It was possible to observe that lactoferrin/curcumin nanoparticles were destabilized during the dynamic digestion process. It was also possible to conclude that low sample volumes can pose a major challenge in the application of dynamic digestion models.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano13152237