Genome-Wide Identification of Detoxification Genes in Wild Silkworm Antheraea pernyi and Transcriptional Response to Coumaphos

For a half-century, the commercial wild silkworm, , has been protected by coumaphos, which is an internal organophosphorus insecticide used to kill the potential parasitic fly larvae inside. Knowledge about the detoxification genes of as well as the detoxification mechanism for this species remains...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (11), p.9775
Main Authors: Chen, Dong-Bin, Xia, Run-Xi, Li, Qun, Li, Yu-Ping, Cao, Hui-Ying, Liu, Yan-Qun
Format: Article
Language:English
Subjects:
ABC transporters
Amino acids
Antheraea pernyi
Chromosomes
Coumaphos
Cytochrome
Detoxification
detoxification gene
differentially expressed genes
Endoplasmic reticulum
Gene set enrichment analysis
Genes
Genomes
Insecticides
Insects
Localization
Metabolism
Organophosphorus pesticides
Phylogenetics
Proteins
Silkworms
Transcriptomes
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Summary:For a half-century, the commercial wild silkworm, , has been protected by coumaphos, which is an internal organophosphorus insecticide used to kill the potential parasitic fly larvae inside. Knowledge about the detoxification genes of as well as the detoxification mechanism for this species remains severely limited. In this study, we identified 281 detoxification genes (32 GSTs, 48 ABCs, 104 CYPs, and 97 COEs) in the genome of this insect, which are unevenly distributed over 46 chromosomes. When compared to the domesticated silkworm, , a lepidopteran model species, has a similar number of ABCs, but a greater number of GSTs, CYPs, and COEs. By transcriptome-based expression analysis, we found that coumaphos at a safe concentration level significantly changed the pathways related to ATPase complex function and the transporter complex in . KEGG functional enrichment analysis indicated that protein processing in the endoplasmic reticulum was the most affected pathway after coumaphos treatment. Finally, we identified four significantly up-regulated detoxification genes ( , , , and ) and one significantly down-regulated detoxification gene ( ) in response to coumaphos treatment, suggesting that these five genes may contribute to detoxification of coumaphos in . Our study provides the first set of detoxification genes for wild silkworms from Saturniidae and highlights the importance of detoxification gene repertoire in insect pesticide tolerance.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119775