Transformative approaches for siRNA detection

• Published in: OpenNano 2024-11, Vol.20, p.100214, Article 100214
• Main Authors: Singh, Sima, Raucci, Ada, Cimmino, Wanda, Miglione, Antonella, Kalligosfyri, Panagiota M, Cinti, Stefano
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Cancer; Optical and electrochemical detection; Precision medicine; siRNA
• ISSN: 2352-9520; 2352-9520
• DOI: 10.1016/j.onano.2024.100214

Small interfering RNA (siRNA) is essential for the process of gene silencing, especially for cancer. Despite its considerable promise, siRNA faces challenges due to stability issues of formulation and undesirable off-target side effects. In order to address these difficulties, it is essential to carefully monitor the levels of siRNA. The existing point-of-care (POC) systems cannot precisely and efficiently detect or monitor siRNA levels. In light of these challenges, this review gives the prospects of siRNA detection by proposing a novel hypothesis of existing electrical and optical-based detection of DNA/RNA with the POC platform. This hypothesis offers an interesting novel perspective to potentially fill the existing gaps, in detecting siRNA. By utilising these technologies, there is high potential to develop a proof-of-concept system that will not only overcome the existing challenges, but it will also allow effective and precise monitoring of siRNA, in real-world healthcare environments. In summary, the prospects for siRNA in the realm of POC platforms are quite encouraging, since it allows precise and effective monitoring. [Display omitted]