Histological and ultrastructural characterization of the dorso-ventral skin of the juvenile and the adult starry puffer fish (Arothron stellatus, Anonymous 1798)

• Published in: BMC veterinary research 2023-10, Vol.19 (1), p.1-221, Article 221
• Main Authors: Madkour, Fatma A, Abdellatif, Ahmed M, Osman, Yassein A, Kandyel, Ramadan M
• Format: Article
• Language: English
• Subjects: Adults; Analysis; Arothron stellatus; Cell cycle; Club cells; Electron microscopes; Electron microscopy; Epidermis; Epithelial cells; Fish; Fisheries; Fishing; Juveniles; Keratinocytes; Microridges; Mucous cells; Oceanography; Scanning electron microscopy; Skin; Spines; Starry puffer fish; Taste buds
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-023-03784-0

Background The starry puffer fish (Arothron stellatus, Anonymous, 1798) is a poisonous tetradontidae fish inhabiting the Red sea. The skin constitutes an important defense against any external effects. The study aims to characterize the dorso-ventral skin of the juvenile and the adult starry puffer fish using light and scanning electron microscopies. Twenty specimens of juvenile and adult fresh fishes were used. Results The scanning electron microarchitecture of the skin of the juvenile and adult fish showed delicate irregular-shaped protrusions, and well-defined bricks-like elevations on the dorsal side and interrupted folds as well as irregular-shaped protrusions on the ventral side. In adult fish, the patterned microridges of the superficial and deep epithelial cells (keratinocytes) were larger and well-defined in the dorsal skin than in the ventral side, the contrary was seen in the juvenile fish. The microridges were arranged in a fingerprint or honeycomb patterns. The openings of the mucous cells were more numerous in the dorsal skin in both age stages but more noticeable in adult. Furthermore, the sensory cells were more dominant in the juveniles than the adults. The odontic spines were only seen in adult. Histologically, few taste buds were observed in the epidermis of the dorsal skin surface of the adult fish. Both mucous and club cells were embedded in the epidermis of the juvenile and adult fish with different shapes and sizes. Melanophores were observed at the dorsal skin of both juvenile and adult fishes while fewer numbers were noticed at the ventral surfaces. Several dermal bony plates with different shapes and sizes were demonstrated in the skin of both adult and juvenile fishes. Conclusion The structural variations of skin of the juvenile and adult fishes may reflect the various environmental difficulties that they confront. Keywords: Club cells, Keratinocytes, Microridges, Mucous cells, Taste buds, Starry puffer fish, Ultrastructure