Algal-Derived Synthesis of Silver Nanoparticles Using the Unicellular ulvophyte sp. MBIC10591: Optimisation, Characterisation, and Biological Activities

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-12, Vol.28 (1), p.279
• Main Authors: Hamida, Reham Samir, Ali, Mohamed Abdelaal, Alkhateeb, Mariam Abdulaziz, Alfassam, Haifa Essa, Momenah, Maha Abdullah, Bin-Meferij, Mashael Mohammed
• Format: Article
• Language: English
• Subjects: Algae; Alzheimer's disease; antibacterial; Antibacterial activity; anticancer; Antiinfectives and antibacterials; Aquatic microorganisms; Bacteria; Biocides; Biological properties; Biomolecules; Biosynthesis; Cancer; Chemical composition; Chloroplasts; Coliforms; Electron microscopy; Fatty acids; Functional groups; Gram-negative bacteria; green synthesis; Hydrocarbons; Kinetics; microalgae; Microorganisms; Morphology; Nanoparticles; optimisation parameter; Phenols; Quantum dots; rRNA 18S; Silver; Stabilizers (agents); Toxicity; Tumors
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28010279

Algal-mediated synthesis of nanoparticles (NPs) is an eco-friendly alternative for producing NPs with potent physicochemical and biological properties. Microalgae represent an ideal bio-nanofactory because they contain several biomolecules acting as passivation and stabilising agents during the biogenesis of NPs. Herein, a novel microalgae sp. was isolated, purified, and identified using light and electron microscopy and 18s rRNA sequencing. The chemical components of their watery extract were assessed using GC-MS. Their dried biomass was used to synthesise silver (Ag) NPs with different optimisation parameters. Ag-NPs were physiochemically characterised, and their anticancer and antibacterial effects were examined. The data showed that the isolated strain was 99% similar to the unicellular sp. MBIC10591; it was ellipsoidal to spherical and had a large cup-shaped spongiomorph chloroplast. The optimum parameters for synthesising Ag-NPs by unicellular sp. MBIC10591 (Uv@Ag-NPs) were as follows: mixture of 1 mM of AgNO with an equal volume of algal extract, 100 °C for 1 h, and pH of 7 under illumination for 24 h. TEM, HRTEM, and SEM revealed that Uv@Ag-NPs are cubic to spherical, with an average nanosize of 12.1 ± 1.2 nm. EDx and mapping analysis showed that the sample had 79% of Ag, while FTIR revealed the existence of several functional groups on the NP surface derivatives from the algal extract. The Uv@Ag-NPs had a hydrodynamic diameter of 178.1 nm and a potential charge of -26.7 mV and showed marked antiproliferative activity against PC3, MDA-MB-231, T47D, and MCF-7, with IC values of 27.4, 20.3, 23.8, and 40 µg/mL, respectively, and moderate toxicity against HFs (IC of 13.3 µg/mL). Uv@Ag-NPs also showed marked biocidal activity against Gram-negative bacteria. was the most sensitive bacteria to the NPs with an inhibition zone of 18.9 ± 0.03 mm. The current study reports, for the first time, the morphological appearance of the novel sp., MBIC10591, and its chemical composition and potential to synthesise Uv@Ag-NPs with smaller sizes and high stability to act as anti-tumour and microbial agents.