Overexpression of HvIcy6 in Barley Enhances Resistance against Tetranychus urticae and Entails Partial Transcriptomic Reprogramming

Cystatins have been largely used for pest control against phytophagous species. However, cystatins have not been commonly overexpressed in its cognate plant species to test their pesticide capacity. Since the inhibitory role of barley HvCPI-6 cystatin against the phytophagous mite has been previousl...

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Published in:International journal of molecular sciences 2018-03, Vol.19 (3), p.697
Main Authors: Santamaria, M Estrella, Diaz-Mendoza, Mercedes, Perez-Herguedas, David, Hensel, Goetz, Kumlehn, Jochen, Diaz, Isabel, Martinez, Manuel
Format: Article
Language:English
Subjects:
Barley
Chemical pest control
cystatin
Cystatins
Gene expression
Genes
Infestation
Metabolism
Pest control
Pesticides
Pests
Photosynthesis
plant defense
Tetranychus urticae
transcriptomics
Transgenes
transgenic
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Summary:Cystatins have been largely used for pest control against phytophagous species. However, cystatins have not been commonly overexpressed in its cognate plant species to test their pesticide capacity. Since the inhibitory role of barley HvCPI-6 cystatin against the phytophagous mite has been previously demonstrated, the purpose of our study was to determine if barley transgenic lines overexpressing its own gene were more resistant against this phytophagous infestation. Besides, a transcriptomic analysis was done to find differential expressed genes among wild-type and transformed barley plants. Barley plants overexpressing cystatin gene remained less susceptible to attack when compared to wild-type plants, with a significant lesser foliar damaged area and a lower presence of the mite. Transcriptomic analysis revealed a certain reprogramming of cellular metabolism and a lower expression of several genes related to photosynthetic activity. Therefore, although caution should be taken to discard potential deleterious pleiotropic effects, cystatins may be used as transgenes with impact on agricultural crops by conferring enhanced levels of resistance to phytophagous pests.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19030697