First Report of Curtobacterium flaccumfaciens in Bulgaria

This study aims at the identification and characterization of five actinobacterial strains with presumed belonging to the species isolated from tomato and pepper plants, and establishing the potential role of both plants as natural reservoirs of this phytopathogen. Species identification was perform...

Full description

Saved in:
Bibliographic Details
Published in:Pathogens (Basel) 2024-06, Vol.13 (6), p.483
Main Authors: Kizheva, Yoana, Pandova, Maria, Dimitrova, Melani, Gladicheva, Yoana, Garkova, Maria, Pirnareva, Desislava, Donchev, Deyan, Moncheva, Penka, Hristova, Petya
Format: Article
Language:English
Subjects:
alternate host plants
antimicrobial substances
Bacteria
Casein
Catalase
Cell size
Curtobacterium flaccumfaciens
Cytochrome
Defoliation
Disease
Esculin
Nitrates
Pathogens
phytopathogenic coryneform bacteria
Prokaryotes
Reservoirs
rRNA 16S
Strains (organisms)
Tomatoes
Urease
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aims at the identification and characterization of five actinobacterial strains with presumed belonging to the species isolated from tomato and pepper plants, and establishing the potential role of both plants as natural reservoirs of this phytopathogen. Species identification was performed via MALDI-ToF MS, 16S rDNA sequencing and PCR. The strains were Gram-positive with a coryneform cell shape having yellow/orange-pigmented colonies; positive for catalase and esculin, and starch and casein hydrolysis; oxidase-, urease-, indole- and nitrate-reduction-negative and were strictly aerobic. All isolates produced antimicrobial substances against various phytopathogenic bacteria. Tomato and pepper plants were artificially infected with newly isolated strains in order to establish their role as natural reservoirs of the bacteria. Morphological alterations were observed only in the tomato plants, with defoliation of the first two to four leaves at the 28th day. Then, viable coryneform bacterial isolates (n = 73) were successfully re-isolated only from the stems of the infected plants. The similarity between the re-isolates and the respective initial isolates was confirmed phenotypically and genotypically by RAPD-PCR, confirming that solanaceous vegetables can act as reservoirs of . This is the first report of in Bulgaria.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens13060483