Circular RNA F-circEA-2a derived from EML4-ALK fusion gene promotes cell migration and invasion in non-small cell lung cancer

Oncogenic fusion gene Echinoderm Microtubule-associated protein-Like 4-Anaplastic Lymphoma Kinase (EML4-ALK) contributes to tumorigenesis of a subset of non-small cell lung cancer (NSCLC). Recently, we demonstrated that F-circEA-4a, a tumor-promoting circular RNA (circRNA) generated from the back-sp...

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Bibliographic Details
Published in:Molecular cancer 2018-09, Vol.17 (1), p.138-138, Article 138
Main Authors: Tan, Shuangyan, Sun, Dan, Pu, Wenchen, Gou, Qiheng, Guo, Chenglin, Gong, Youling, Li, Jiao, Wei, Yu-Quan, Liu, Lunxu, Zhao, Yun, Peng, Yong
Format: Article
Language:English
Subjects:
ALK protein
Biomarkers
Biopsy
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - pathology
Care and treatment
Cell adhesion & migration
Cell fusion
Cell growth
Cell Line, Tumor
Cell migration
Cell migration/invasion
Cell Movement - genetics
Cell Proliferation
Chromosome Breakpoints
Circular RNA
Cytoplasm
Diagnosis
EML4-ALK
Fusion protein
Genetic aspects
Humans
Kinases
Laboratories
Letter to the Editor
Lung cancer
Lung cancer, Non-small cell
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Lymphoma
Non-small cell lung cancer
Non-small cell lung carcinoma
Oncogene Proteins, Fusion - genetics
Patient outcomes
Protein-tyrosine kinase
Proteins
Ribonucleic acid
RNA
RNA - genetics
Sequence Analysis, DNA
Splicing
Thoracic surgery
Tumorigenesis
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Summary:Oncogenic fusion gene Echinoderm Microtubule-associated protein-Like 4-Anaplastic Lymphoma Kinase (EML4-ALK) contributes to tumorigenesis of a subset of non-small cell lung cancer (NSCLC). Recently, we demonstrated that F-circEA-4a, a tumor-promoting circular RNA (circRNA) generated from the back-splicing of EML4-ALK variant 3b (v3b), is a novel liquid biopsy biomarker for NSCLC. However, circRNAs produced from EML4-ALK gene and their roles in NSCLC are not well-characterized. Here, we identify another EML4-ALK-v3b-derived circRNA, F-circEA-2a, harboring "AA" (rather than "AAAA" in F-circEA-4a) motif at the junction site. F-circEA-2a mainly locates in the cytoplasm and promotes cell migration and invasion, but has little effect on cell proliferation. Moreover, F-circEA-2a exists in tumor, but not in the plasma of NSCLC patients with EML4-ALK fusion gene, further supporting the significant diagnostic value of F-circEA-4a for EML4-ALK-positive NSCLC. This work finds a novel oncogenic circRNA generated from EML4-ALK fusion gene, highlighting the pivotal role of circRNA in EML4-ALK-positive NSCLC development.
ISSN:1476-4598
1476-4598
DOI:10.1186/s12943-018-0887-9