Evaluation of the Cytotoxicity of Ayahuasca Beverages

Ayahuasca is a beverage consumed at shamanic ceremonies and currently has gained popularity on recreational scenarios. It contains beta-carboline alkaloids and , -dimethyltryptamine, which possesses hallucinogenic effects. Only a few studies have elicited the psychoactive effects and the dose of suc...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2020-11, Vol.25 (23), p.5594
Main Authors: Simão, Ana Y, Gonçalves, Joana, Gradillas, Ana, García, Antonia, Restolho, José, Fernández, Nicolás, Rodilla, Jesus M, Barroso, Mário, Duarte, Ana Paula, Cristóvão, Ana C, Gallardo, Eugenia
Format: Article
Language:English
Subjects:
Alkaloids
Animals
Apoptosis
Apoptosis - drug effects
ayahuasca
Banisteriopsis - chemistry
Banisteriopsis caapi
beta-carboline alkaloids
Beverages
Beverages - analysis
Cell lines
Cell viability
Cells, Cultured
Cytotoxicity
Cytotoxins - pharmacology
Dopamine
Dopamine receptors
Dopaminergic Neurons - drug effects
Dopaminergic Neurons - pathology
Hallucinogens
mesencephalic dopaminergic neurons
Mesencephalon - drug effects
Mesencephalon - pathology
Mimosa tenuiflora
N,N-dimethyltryptamine
Peganum harmala
Plant Extracts - pharmacology
Proteins
Psychotria viridis
Psychotropic drugs
Rats
Tea
Toxicity
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Summary:Ayahuasca is a beverage consumed at shamanic ceremonies and currently has gained popularity on recreational scenarios. It contains beta-carboline alkaloids and , -dimethyltryptamine, which possesses hallucinogenic effects. Only a few studies have elicited the psychoactive effects and the dose of such compounds on neurological dopaminergic cells or animals. In this work, we aimed to study the cytotoxic effects of these compounds present in ayahuasca beverages and on five different teas ( , , , and Dc Ab (commercial name)) preparations on dopaminergic immortalized cell lines. Moreover, a characterization of the derivative alkaloids was also performed. All the extracts were characterized by chromatographic systems and the effect of those compounds in cell viability and total protein levels were analyzed in N27 dopaminergic neurons cell line. This is the first article where cytotoxicity of ayahuasca tea is studied on neurological dopaminergic cells. Overall, results showed that both cell viability and protein contents decreased when cells were exposed to the individual compounds, as well as to the teas and to the two mixtures based on the traditional ayahuasca beverages.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules25235594