Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803

Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of -acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In...

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Bibliographic Details
Published in:Bio-protocol 2017-03, Vol.7 (6), p.e2176-e2176
Main Authors: Galmozzi, Carla V, Muro-Pastor, M Isabel
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of -acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In this protocol, we describe a procedure for the isolation of 70S ribosomes from the unicellular cyanobacterium sp. PCC 6803 (hereafter ). We have successfully used this protocol in our study of the cyanobacterial ribosomal-associated protein LrtA, which is a homologue of bacterial HPF (hibernation promoting factor) ( Galmozzi , 2016 ).
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2176