Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

• Published in: Onderstepoort journal of veterinary research 2019-08, Vol.86 (1), p.1-8
• Main Authors: Timurkan, Mehmet O., Polat, Pelin F., Şahan, Adem, Dinçer, Ender, Aksoy, Gürbüz
• Format: Article
• Language: English
• Subjects: Agriculture, Dairy & Animal Science; Amino acids; Amplified Fragment Length Polymorphism Analysis - veterinary; Anatomy & Morphology; Animals; Anorexia; Biochemistry & Molecular Biology; Canine parvovirus; Canine Parvovirus variants; Causes of; Deoxyribonucleic acid; Diarrhea; DNA; Dog diseases; Dog Diseases - virology; Dogs; Evolution & development; Gene polymorphism; Genes; Genetic aspects; Genetic polymorphisms; Genetic research; Genomes; Genomics; Health aspects; Identification and classification; Immunization; Immunology; Molecular characterisation; Mutation; Original Research; Parvoviridae Infections - veterinary; Parvoviridae Infections - virology; Parvovirus infections; Parvovirus, Canine - classification; Parvovirus, Canine - genetics; Parvovirus, Canine - isolation & purification; Parvoviruses; Pharmacology & Pharmacy; Phylogeny; Physiology; Polymerase chain reaction; Polymerase Chain Reaction - veterinary; Polymorphism; Polymorphism, Restriction Fragment Length; Proteins; Restriction fragment length polymorphism; RFLP; Studies; Turkey; Vaccines; Veterinary medicine; Veterinary Sciences; Viral diseases; Virus diseases; Viruses; Vomiting
• ISSN: 0030-2465; 2219-0635; 2219-0635
• DOI: 10.4102/ojvr.v86i1.1734

Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV- 2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey’s dog population.