Profiling of Polyphenolic Compounds of Leontopodium alpinum Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects

Cass. (edelweiss) is recognized as a frequent constituent of anti-aging skin care products, providing increased antioxidant and anti-inflammatory defense. Considering the growing demand and the protected status of edelweiss in many countries, alternative methods of production have been developed, on...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2021-12, Vol.11 (1), p.100
Main Authors: Pralea, Ioana-Ecaterina, Moldovan, Radu-Cristian, Țigu, Adrian-Bogdan, Petrache, Alina-Maria, Hegheș, Simona-Codruța, Mitoi, Monica, Cogălniceanu, Gina, Iuga, Cristina-Adela
Format: Article
Language:English
Subjects:
Acids
Aging
Antioxidants
Apoptosis
Breast cancer
Callus
callus cultures
Colon
Colon cancer
Constituents
edelweiss
Free radicals
Inflammation
ion-mobility mass spectrometry
Ionic mobility
Liquid chromatography
Lung cancer
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Morphology
phytochemical characterization
Phytochemicals
Polyphenols
Production methods
Prostate
Scavenging
Screening
Tumor cell lines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cass. (edelweiss) is recognized as a frequent constituent of anti-aging skin care products, providing increased antioxidant and anti-inflammatory defense. Considering the growing demand and the protected status of edelweiss in many countries, alternative methods of production have been developed, one of them being callus culturing. This study reports the phytochemical composition of a methanolic extract of callus cultures, characterized by liquid chromatography coupled to ion-mobility high resolution mass spectrometry (UPLC/IM-HRMS). The methanolic extract exhibited strong free radical scavenging activity (122.19 ± 7.28 mg AAE/g dw), while the quantitative evaluation revealed that four major constituents (phenylpropanoid derivatives) represent 57.13% (m/m) of the extract. Consequently, a screening of antiproliferative effects was performed on ten cancer cell lines, representative of prostate, colon, lung and breast cancer, showing inhibition of colony formation in all cases. These results provide a comprehensive phytochemical characterization of callus cultures using advanced IM-HRMS, while the in vitro explorations confirmed the potent antioxidant properties of edelweiss which are worth exploring further in cancer prevention.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11010100