Complex of Defense Polypeptides of Wheatgrass ( Elytrigia elongata ) Associated with Plant Immunity to Biotic and Abiotic Stress Factors

• Published in: Plants (Basel) 2024-09, Vol.13 (17), p.2459
• Main Authors: Barashkova, Anna S, Smirnov, Alexey N, Rogozhin, Eugene A
• Format: Article
• Language: English
• Subjects: Abiotic factors; Abiotic stress; Antifungal activity; Antimicrobial agents; Biological activity; biotic and abiotic stresses; Biotic factors; Cereals; Defense mechanisms; defense peptides; Defensins; Disease resistance; Elongation; Elytrigia; Environmental factors; Evolution; Fractionation; Fungi; Fungicides; Immunity (Disease); Lipids; Peptides; Physiology; Plant extracts; Plant immunity; Plant layout; Plants; Polypeptides; Proteins; reactivating effect; Salinity; Stress concentration; structure analysis; Trypsin; Trypsin inhibitors; Wheat; Yeast; α-Amylase
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13172459

Plant defense polypeptides play a crucial role in providing plants with constitutive immunity against various biotic and abiotic stressors. In this study, we explored a complex of proteins from wheatgrass ( ) spikelets to estimate their role in the plant's tolerance to various environmental factors. The current research shows that in vitro protein extracts from spikelets possess antifungal activity against certain species, which are specific cereal pathogens, at concentrations of 1-2 mg/mL. In this study, we reproduced these antifungal activities using a 4 mg/mL extract in artificial fungal infection experiments on wheat grain ( ) under controlled laboratory conditions. Furthermore, the tested extract demonstrated a protective effect on exposed to hyper-salinity stress at a concentration of 2 mg/mL. A combined scheme of fractionation and structural identification was applied for the estimation of the diversity of defense polypeptides. Defensins, lipid-transfer proteins, hydrolase inhibitors (cereal bifunctional trypsin/alpha-amylase inhibitors from a Bowman-Birk trypsin inhibitor), and high-molecular-weight disease resistance proteins were isolated from the extract. Thus, wheatgrass spikelets appear to be a reservoir of defense polypeptides. Our findings contribute to a deeper understanding of plant defense proteins and peptides and their involvement in the adaptation to various stress factors, and they reveal the regulatory effect at the ecosystem level.