Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

• Published in: Journal of advanced veterinary and animal research 2024-03, Vol.11 (1), p.1-8
• Main Authors: Alam, Muhammad Ashiqul, Hossain, Md Jannat, Sohidullah, M, Khan, Md Shahidur Rahman, Islam, Khan Md Shaiful
• Format: Article
• Language: English
• Subjects: Bacteria; bacteria; cattle; cellulolytic; rumen; ruminococcus spp; Carbon dioxide; Catalase; Cattle; Cellobiose; Cellulose; Efficiency; Fatty acids; Feed conversion; Feed efficiency; Feeds; Fermentation; Gelatin; Glucose; Gram-positive cocci; Inoculum; Lactose; Liquefaction; Metabolism; Morphology; Nutritive value; Original; Physiology; Poultry; Poultry feed; Redox potential; Rice; Rice bran; Rumen; Ruminococcus; Xylan
• ISSN: 2311-7710; 2311-7710
• DOI: 10.5455/javar.2024.k740

To isolate and characterize cellulolytic rumen bacteria from the rumen of Sahiwal cattle using rumen bacterial inoculum to increase the nutritional value of rice bran used as broiler feed. The ruminal liquid was kept at an optimal pH of 6.9 and a redox potential of less than -300 mV while being incubated anaerobically at 39°C in a medium containing rumen fluid glucose cellobiose agar. By using the Hungate technique, the organisms were detected based on their morphological, physiological, biochemical, and molecular testing. The findings revealed that the isolated , and were obligate anaerobic, generally Gram-positive, nonmotile cocci or rod, single or pair, occasionally short chain, producing yellow pigment when grown on cellulose, and having a clear zone around the colonies. Both isolate fermented sugars such as cellobiose, glucose, and lactose, as well as decomposed xylan. The results also showed that the isolates recognized as spp., a cellulolytic rumen bacterium, were catalase-negative, indole-negative, and gelatin liquefaction-positive. Isolation and characterization of spp. may be helpful for Bangladesh in reducing the cost of producing poultry feed and circumventing restrictions on rice bran use. We can also develop more efficient and long-lasting plans to enhance poultry performance and feed efficiency, as well as increase the nutritional value of rice bran used as broiler feed, by understanding how various spp. function in this process.