Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in Capsicum annuum and Characterization of CaAnn9 in Salt Tolerance

Annexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of CaAnn genes of pepper (Capsicum annuum) at the genome-wide level is li...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (16), p.8667
Main Authors: Wu, Xiaoxia, Ren, Yan, Jiang, Hailong, Wang, Yan, Yan, Jiaxing, Xu, Xiaoying, Zhou, Fucai, Ding, Haidong
Format: Article
Language:English
Subjects:
Abiotic stress
Abscisic acid
annexin
Antioxidants
Binding sites
Biodegradation
bioinformatic analysis
CaAnn9
Calcium-binding protein
Candidates
Capsicum annuum
Chlorophyll
Cloning
Cold
Cotton
Drought
Gene expression
Gene silencing
Genes
Genomes
Hormones
oxidative damage
pepper
Peppers
Phospholipids
Phylogenetics
Phylogeny
Plant growth
Polygenic inheritance
Polymerase chain reaction
Proteins
Reactive oxygen species
Regulation
Salinity tolerance
salt stress
Seedlings
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Summary:Annexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of CaAnn genes of pepper (Capsicum annuum) at the genome-wide level is limited. Based on the available pepper genomic information, we identified 15 members of the CaAnn gene family. Phylogenetic analysis showed that CaAnn proteins could be categorized into four different orthologous groups. Real time quantitative RT-PCR analysis showed that the CaAnn genes were tissue-specific and were widely expressed in pepper leaves after treatments with cold, salt, and drought, as well as exogenously applied MeJA and ABA. In addition, the function of CaAnn9 was further explored using the virus-induced gene silencing (VIGS) technique. CaAnn9-silenced pepper seedlings were more sensitive to salt stress, reflected by the degradation of chlorophyll, the accumulation of reactive oxygen species (ROS), and the decrease of antioxidant defense capacity. This study provides important information for further study of the role of pepper CaAnn genes and their coding proteins in growth, development, and environmental responses.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22168667