Ca2+ imaging with two-photon microscopy to detect the disruption of brain function in mice administered neonicotinoid insecticides

Neonicotinoid pesticides are a class of insecticides that reportedly have harmful effects on bees and dragonflies, causing a reduction in their numbers. Neonicotinoids act as neuroreceptor modulators, and some studies have reported their association with neurodevelopmental disorders. However, the pr...

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Bibliographic Details
Published in:Scientific reports 2022-03, Vol.12 (1), p.5114-5114, Article 5114
Main Authors: Hirai, Anri, Sugio, Shouta, Nimako, Collins, Nakayama, Shouta M. M., Kato, Keisuke, Takahashi, Keisuke, Arizono, Koji, Hirano, Tetsushi, Hoshi, Nobuhiko, Fujioka, Kazutoshi, Taira, Kumiko, Ishizuka, Mayumi, Wake, Hiroaki, Ikenaka, Yoshinori
Format: Article
Language:English
Subjects:
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Acetylcholine receptors (nicotinic)
Anxiety
Body weight
Calcium imaging
Central nervous system
Humanities and Social Sciences
Insecticides
Lethal dose
Metabolites
multidisciplinary
Neurodevelopmental disorders
Neuroimaging
Neuromodulation
Nicotine
Oral administration
Pesticides
Science
Science (multidisciplinary)
Somatosensory cortex
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Summary:Neonicotinoid pesticides are a class of insecticides that reportedly have harmful effects on bees and dragonflies, causing a reduction in their numbers. Neonicotinoids act as neuroreceptor modulators, and some studies have reported their association with neurodevelopmental disorders. However, the precise effect of neonicotinoids on the central nervous system has not yet been identified. Herein, we conducted in vivo Ca 2+ imaging using a two-photon microscope to detect the abnormal activity of neuronal circuits in the brain after neonicotinoid application. The oral administration of acetamiprid (ACE) (20 mg/kg body weight (BW) in mature mice with a quantity less than the no-observed-adverse-effect level (NOAEL) and a tenth or half of the median lethal dose (LD 50 ) of nicotine (0.33 or 1.65 mg/kg BW, respectively), as a typical nicotinic acetylcholine receptor (nAChR) agonist, increased anxiety-like behavior associated with altered activities of the neuronal population in the somatosensory cortex. Furthermore, we detected ACE and its metabolites in the brain, 1 h after ACE administration. The results suggested that in vivo Ca 2+ imaging using a two-photon microscope enabled the highly sensitive detection of neurotoxicant-mediated brain disturbance of nerves.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-09038-7