Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice

Postoperative neurocognitive impairment is an urgent problem with global aging accelerating. The prevention and treatment of postoperative neurocognitive impairment have been widely investigated but lack effective strategies. Low-intensity pulsed ultrasound (LIPUS), a non-invasive tool, has shown an...

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Bibliographic Details
Published in:Brain sciences 2023-04, Vol.13 (4), p.657
Main Authors: Wang, Qian, Liu, Taotao, Chang, Huixian, Li, Zhengqian, Chen, Lei, Mi, Xinning, Xing, Huayi, Wang, Xiaoxiao, Hong, Jingshu, Liu, Kaixi, Li, Yitong, Han, Dengyang, Li, Yue, Yang, Ning, Li, Xiaoli, Li, Yingwei, Guo, Xiangyang
Format: Article
Language:English
Subjects:
Abdomen
Analysis
Animal cognition
Brain
Brain research
Calpain1
Cognition
Cognition disorders
Cognitive ability
delayed neurocognitive recovery (dNCR)
Hippocampus
Intervention
Laboratory animals
Laparotomy
low-intensity pulsed ultrasound (LIPUS)
Neuroprotection
Phosphorylation
Piezo1
Protein kinases
Proteins
Surgery
Synaptogenesis
Ultrasonic imaging
Ultrasound
Western blotting
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Summary:Postoperative neurocognitive impairment is an urgent problem with global aging accelerating. The prevention and treatment of postoperative neurocognitive impairment have been widely investigated but lack effective strategies. Low-intensity pulsed ultrasound (LIPUS), a non-invasive tool, has shown an effect on neuroprotection, but whether it could attenuate the postoperative neurocognitive impairment and the underlying mechanisms remains unknown. An experimental setup for LIPUS stimulation of the hippocampus was well established. A laparotomy model in aged mice was applied, and a Morris water maze was used to assess cognitive function. RT-qPCR and western blotting were used to detect levels of Piezo1, synapse-associated proteins in the hippocampus, respectively. Immunofluorescent staining was also used to determine the neural activation and Piezo1 expression. The results showed that LIPUS increased synapse-related proteins of the hippocampus and attenuated cognitive impairment in aged mice. Meanwhile, LIPUS suppressed the overexpression of Piezo1 in the hippocampus. We further found that LIPUS promoted Calpain1 activity and increased extracellular regulated protein kinases (Erk) phosphorylation. Our results suggested that LIPUS could improve cognitive impairment and increase hippocampal synaptogenesis through the Piezo1-mediated Calpain1/ Erk pathway. LIPUS could be used as an effective physical intervention to alleviate postoperative cognitive dysfunction in the aged population.
ISSN:2076-3425
2076-3425
DOI:10.3390/brainsci13040657