Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells

We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was block...

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Bibliographic Details
Published in:Journal of Pharmacological Sciences 2010, Vol.112(2), pp.247-250
Main Authors: Okubo, Migiwa, Kawaguchi, Mitsuru
Format: Article
Language:English
Subjects:
amylase release
Amylases - metabolism
Animals
Bicuculline - metabolism
Clonazepam - pharmacology
Diazepam - pharmacology
Isoproterenol - pharmacology
Male
Muscimol - pharmacology
parotid gland
Parotid Gland - cytology
Parotid Gland - metabolism
Rats
Rats, Wistar
Receptors, GABA-A - drug effects
Receptors, GABA-A - metabolism
γ-aminobutyric acid A receptor
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Summary:We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was blocked by bicuculline, a GABA A-receptor antagonist, and enhanced by clonazepam, a central-type benzodiazepine-receptor agonist, and diazepam, a central- and peripheral-type benzodiazepine-receptor agonist. Although bicuculline completely blocked the combination effect of GABA A-receptor agonist and clonazepam, it did not completely block the combination effect with diazepam. These results suggest that protein secretion is suppressively regulated by GABA A receptors coupled with central-type benzodiazepine receptors.
ISSN:1347-8613
1347-8648
DOI:10.1254/jphs.09269SC