Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells

We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was block...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Pharmacological Sciences 2010, Vol.112(2), pp.247-250
Main Authors: Okubo, Migiwa, Kawaguchi, Mitsuru
Format: Article
Language:English
Subjects:
amylase release
Amylases - metabolism
Animals
Bicuculline - metabolism
Clonazepam - pharmacology
Diazepam - pharmacology
Isoproterenol - pharmacology
Male
Muscimol - pharmacology
parotid gland
Parotid Gland - cytology
Parotid Gland - metabolism
Rats
Rats, Wistar
Receptors, GABA-A - drug effects
Receptors, GABA-A - metabolism
γ-aminobutyric acid A receptor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443
cites cdi_FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443
container_end_page 250
container_issue 2
container_start_page 247
container_title Journal of Pharmacological Sciences
container_volume 112
creator Okubo, Migiwa
Kawaguchi, Mitsuru
description We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was blocked by bicuculline, a GABA A-receptor antagonist, and enhanced by clonazepam, a central-type benzodiazepine-receptor agonist, and diazepam, a central- and peripheral-type benzodiazepine-receptor agonist. Although bicuculline completely blocked the combination effect of GABA A-receptor agonist and clonazepam, it did not completely block the combination effect with diazepam. These results suggest that protein secretion is suppressively regulated by GABA A receptors coupled with central-type benzodiazepine receptors.
doi_str_mv 10.1254/jphs.09269SC
format article
fullrecord <record><control><sourceid>elsevier_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_044256dabf1b461890f671c754eae6ec</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S134786131931028X</els_id><doaj_id>oai_doaj_org_article_044256dabf1b461890f671c754eae6ec</doaj_id><sourcerecordid>S134786131931028X</sourcerecordid><originalsourceid>FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443</originalsourceid><addsrcrecordid>eNptkUFu3CAUhq2qVZOm3XVdcYA6BRvDeOlOkjZSpFZpukYYHgkjD1hAUk3u0ZPkHj1T8XhiddENoMfHx4O_KN4TfEqqhn7ajHfxFLcVa3-sXxTHpKa8XDG6ermsSX1UvIlxg3G1woS9Lo4qTEius-Pi99qXfoQgk30AdG4MqIQ-Q_oF4NCfp7LbWuf7-7QLVqFOWY06dA0KxuRDRNJptAaXghzKm90I-aR79NrKRxitg39I71C33Q0yTsUBptk6dC0T-i6DT5NXWSdD1g1DfFu8MnKI8O4wnxQ_L85v1l_Lq29fLtfdVakY4amEltG6xrxiBjjhPWCKMasob0FjKYERYA2lNeFAsTZG16sWQ80b03BM88ZJcTl7tZcbMQa7lWEnvLRiX_DhVsiQrBpAZL5qmJa9IT1lJIsM40TxhkK-CFR2fZxdKvgYA5jFR7CYkhJTUuKQVMY_zPh4329BL_BzNBk4m4FNTPIWFuC5ob2NkEpU-_HgXbbVnQwCXNawWQP5Hx8sBBGVBadA25Czzg-1_2_wL3oLu4g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells</title><source>ScienceDirect (Online service)</source><creator>Okubo, Migiwa ; Kawaguchi, Mitsuru</creator><creatorcontrib>Okubo, Migiwa ; Kawaguchi, Mitsuru</creatorcontrib><description>We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was blocked by bicuculline, a GABA A-receptor antagonist, and enhanced by clonazepam, a central-type benzodiazepine-receptor agonist, and diazepam, a central- and peripheral-type benzodiazepine-receptor agonist. Although bicuculline completely blocked the combination effect of GABA A-receptor agonist and clonazepam, it did not completely block the combination effect with diazepam. These results suggest that protein secretion is suppressively regulated by GABA A receptors coupled with central-type benzodiazepine receptors.</description><identifier>ISSN: 1347-8613</identifier><identifier>EISSN: 1347-8648</identifier><identifier>DOI: 10.1254/jphs.09269SC</identifier><identifier>PMID: 20118616</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>amylase release ; Amylases - metabolism ; Animals ; Bicuculline - metabolism ; Clonazepam - pharmacology ; Diazepam - pharmacology ; Isoproterenol - pharmacology ; Male ; Muscimol - pharmacology ; parotid gland ; Parotid Gland - cytology ; Parotid Gland - metabolism ; Rats ; Rats, Wistar ; Receptors, GABA-A - drug effects ; Receptors, GABA-A - metabolism ; γ-aminobutyric acid A receptor</subject><ispartof>Journal of Pharmacological Sciences, 2010, Vol.112(2), pp.247-250</ispartof><rights>2010 Elsevier B.V.</rights><rights>The Japanese Pharmacological Society 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443</citedby><cites>FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S134786131931028X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,4024,27923,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20118616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okubo, Migiwa</creatorcontrib><creatorcontrib>Kawaguchi, Mitsuru</creatorcontrib><title>Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells</title><title>Journal of Pharmacological Sciences</title><addtitle>J Pharmacol Sci</addtitle><description>We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was blocked by bicuculline, a GABA A-receptor antagonist, and enhanced by clonazepam, a central-type benzodiazepine-receptor agonist, and diazepam, a central- and peripheral-type benzodiazepine-receptor agonist. Although bicuculline completely blocked the combination effect of GABA A-receptor agonist and clonazepam, it did not completely block the combination effect with diazepam. These results suggest that protein secretion is suppressively regulated by GABA A receptors coupled with central-type benzodiazepine receptors.</description><subject>amylase release</subject><subject>Amylases - metabolism</subject><subject>Animals</subject><subject>Bicuculline - metabolism</subject><subject>Clonazepam - pharmacology</subject><subject>Diazepam - pharmacology</subject><subject>Isoproterenol - pharmacology</subject><subject>Male</subject><subject>Muscimol - pharmacology</subject><subject>parotid gland</subject><subject>Parotid Gland - cytology</subject><subject>Parotid Gland - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, GABA-A - drug effects</subject><subject>Receptors, GABA-A - metabolism</subject><subject>γ-aminobutyric acid A receptor</subject><issn>1347-8613</issn><issn>1347-8648</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNptkUFu3CAUhq2qVZOm3XVdcYA6BRvDeOlOkjZSpFZpukYYHgkjD1hAUk3u0ZPkHj1T8XhiddENoMfHx4O_KN4TfEqqhn7ajHfxFLcVa3-sXxTHpKa8XDG6ermsSX1UvIlxg3G1woS9Lo4qTEius-Pi99qXfoQgk30AdG4MqIQ-Q_oF4NCfp7LbWuf7-7QLVqFOWY06dA0KxuRDRNJptAaXghzKm90I-aR79NrKRxitg39I71C33Q0yTsUBptk6dC0T-i6DT5NXWSdD1g1DfFu8MnKI8O4wnxQ_L85v1l_Lq29fLtfdVakY4amEltG6xrxiBjjhPWCKMasob0FjKYERYA2lNeFAsTZG16sWQ80b03BM88ZJcTl7tZcbMQa7lWEnvLRiX_DhVsiQrBpAZL5qmJa9IT1lJIsM40TxhkK-CFR2fZxdKvgYA5jFR7CYkhJTUuKQVMY_zPh4329BL_BzNBk4m4FNTPIWFuC5ob2NkEpU-_HgXbbVnQwCXNawWQP5Hx8sBBGVBadA25Czzg-1_2_wL3oLu4g</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Okubo, Migiwa</creator><creator>Kawaguchi, Mitsuru</creator><general>Elsevier B.V</general><general>The Japanese Pharmacological Society</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>2010</creationdate><title>Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells</title><author>Okubo, Migiwa ; Kawaguchi, Mitsuru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>amylase release</topic><topic>Amylases - metabolism</topic><topic>Animals</topic><topic>Bicuculline - metabolism</topic><topic>Clonazepam - pharmacology</topic><topic>Diazepam - pharmacology</topic><topic>Isoproterenol - pharmacology</topic><topic>Male</topic><topic>Muscimol - pharmacology</topic><topic>parotid gland</topic><topic>Parotid Gland - cytology</topic><topic>Parotid Gland - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, GABA-A - drug effects</topic><topic>Receptors, GABA-A - metabolism</topic><topic>γ-aminobutyric acid A receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okubo, Migiwa</creatorcontrib><creatorcontrib>Kawaguchi, Mitsuru</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Journal of Pharmacological Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okubo, Migiwa</au><au>Kawaguchi, Mitsuru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells</atitle><jtitle>Journal of Pharmacological Sciences</jtitle><addtitle>J Pharmacol Sci</addtitle><date>2010</date><risdate>2010</risdate><volume>112</volume><issue>2</issue><spage>247</spage><epage>250</epage><pages>247-250</pages><issn>1347-8613</issn><eissn>1347-8648</eissn><abstract>We investigated the inhibitory role of γ-aminobutyric acid A (GABA A ) receptors on amylase release and the evidence for functional coupling with central-type benzodiazepine receptors in rat parotid acinar cells. Muscimol and GABA decreased isoprenaline-induced amylase release. This effect was blocked by bicuculline, a GABA A-receptor antagonist, and enhanced by clonazepam, a central-type benzodiazepine-receptor agonist, and diazepam, a central- and peripheral-type benzodiazepine-receptor agonist. Although bicuculline completely blocked the combination effect of GABA A-receptor agonist and clonazepam, it did not completely block the combination effect with diazepam. These results suggest that protein secretion is suppressively regulated by GABA A receptors coupled with central-type benzodiazepine receptors.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>20118616</pmid><doi>10.1254/jphs.09269SC</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1347-8613
ispartof Journal of Pharmacological Sciences, 2010, Vol.112(2), pp.247-250
issn 1347-8613
1347-8648
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_044256dabf1b461890f671c754eae6ec
source ScienceDirect (Online service)
subjects amylase release
Amylases - metabolism
Animals
Bicuculline - metabolism
Clonazepam - pharmacology
Diazepam - pharmacology
Isoproterenol - pharmacology
Male
Muscimol - pharmacology
parotid gland
Parotid Gland - cytology
Parotid Gland - metabolism
Rats
Rats, Wistar
Receptors, GABA-A - drug effects
Receptors, GABA-A - metabolism
γ-aminobutyric acid A receptor
title Co-operative Effect Between γ-Aminobutyric Acid A Receptors and Central-Type Benzodiazepine Receptors on Amylase Release in Rat Parotid Acinar Cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T20%3A48%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Co-operative%20Effect%20Between%20%CE%B3-Aminobutyric%20Acid%20A%20Receptors%20and%20Central-Type%20Benzodiazepine%20Receptors%20on%20Amylase%20Release%20in%20Rat%20Parotid%20Acinar%20Cells&rft.jtitle=Journal%20of%20Pharmacological%20Sciences&rft.au=Okubo,%20Migiwa&rft.date=2010&rft.volume=112&rft.issue=2&rft.spage=247&rft.epage=250&rft.pages=247-250&rft.issn=1347-8613&rft.eissn=1347-8648&rft_id=info:doi/10.1254/jphs.09269SC&rft_dat=%3Celsevier_doaj_%3ES134786131931028X%3C/elsevier_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c617t-e964330726fe717be040062479ed0aae61e6544317e40dffd3890e375f5704443%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/20118616&rfr_iscdi=true