Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p

• Published in: BMC nephrology 2024-04, Vol.25 (1), p.130-130, Article 130
• Main Authors: Kuang, Yongling, Yang, Juan, Sun, Meimei, Rui, Tingting, Yang, Zhenhua, Shi, Meihua
• Format: Article
• Language: English
• Subjects: Analysis; Antibodies; Apoptosis; Biomarkers; Cancer; Care and treatment; Cell growth; Cell proliferation; DANCR; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2; Diabetic nephropathies; Diabetic Nephropathies - genetics; Diabetic nephropathy; Diagnosis; Diagnostic; Enzyme-linked immunosorbent assay; Epithelial cells; Flow cytometry; Glucose; Humans; Immunoprecipitation; Inflammation; Kidney diseases; Kidney failure; Kidneys; KLF5; Krueppel-like factor; Kruppel-Like Transcription Factors - genetics; Laboratories; MicroRNA; MicroRNAs; MicroRNAs - genetics; miR-214-5p; miRNA; Nephropathy; Non-coding RNA; Oxidative stress; Polymerase chain reaction; Prevention; Proteins; Reactive oxygen species; Reverse transcription; Ribonucleic acid; Risk factors; RNA; RNA, Long Noncoding - genetics; Testing; Transcription Factors
• ISSN: 1471-2369; 1471-2369
• DOI: 10.1186/s12882-024-03562-6

Diabetic nephropathy (DN) manifests a critical aspect in the form of renal tubular injury. The current research aimed to determine the function and mechanism of long non-coding ribonucleic acid (LncRNA) differentiation antagonising non-protein coding RNA (DANCR), with a focus on its impact on renal tubular injury. Quantitative reverse transcription polymerase chain reaction was employed to analyze the RNA levels of DANCR in the serum of patients with DN or human proximal tubular epithelial cells (human kidney 2 [HK2]). The diagnostic significance of DANCR was assessed using a receiver operating characteristic curve. A DN model was established by inducing HK-2 cells with high glucose (HG). Cell proliferation, apoptosis, and the levels of inflammatory factors, reactive oxygen species (ROS), and malondialdehyde (MDA) were detected using the Cell Counting Kit - 8, flow cytometry, and enzyme-linked immunosorbent assay. The interaction between microRNA (miR)-214-5p and DANCR or Krüppel-like factor 5 (KLF5) was investigated using RNA immunoprecipitation and dual-luciferase reporter assays. Elevated levels of DANCR were observed in the serum of patients with DN and HG-inducted HK-2 cells (P