Autologous peripheral blood stem cell harvest: Collection efficiency and factors affecting it

Harvest of hematopoietic progenitor cells via leukapheresis is being used increasingly for transplants in India. Adequate yield of cells per kilogram body weight of recipient is required for successful engraftment. Collection efficiency (CE) is an objective quality parameter used to assess the quali...

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Bibliographic Details
Published in:Asian journal of transfusion science 2016-01, Vol.10 (1), p.93-97
Main Authors: Tiwari, Aseem K, Pandey, Prashant, Subbaraman, Harini, Bhargava, Rahul, Rawat, Ganesh, Madiraju, Shivani, Raina, Vimarsh, Bhargava, Richa
Format: Article
Language:English
Subjects:
Automation
Bone marrow
Chemotherapy
Cloning
Collection efficiency
Collections
Conflicts of interest
Efficiency
Growth factors
Hematopoietic stem cells
leukapheresis
Mortality
Original
peripheral blood stem cells
Software
Stem cells
Transplants & implants
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Summary:Harvest of hematopoietic progenitor cells via leukapheresis is being used increasingly for transplants in India. Adequate yield of cells per kilogram body weight of recipient is required for successful engraftment. Collection efficiency (CE) is an objective quality parameter used to assess the quality of leukapheresis program. In this study, we calculated the CE of the ComTec cell separator (Fresenius Kabi, Germany) using two different formulae (CE1 and CE2) and analyzed various patient and procedural factors, which may affect it. One hundred and one consecutive procedures in 77 autologous donors carried out over 3 years period were retrospectively reviewed. Various characteristics like gender, age, weight, disease status, hematocrit, preprocedure total leukocyte count, preprocedure CD34 positive (CD34+) cells count, preprocedure absolute CD34+ cell count and processed apheresis volume effect on CE were compared. CE for each procedure was calculated using two different formulae, and results were compared using statistical correlation and regression analysis. The mean CE1 and CE2 was 41.2 and 49.1, respectively. CE2 appeared to be more accurate indicator of overall CE as it considered the impact of continued mobilization of stem cells during apheresis procedure, itself. Of all the factors affecting CE, preprocedure absolute CD34+ was the only independent factor affecting CE. The only factor affecting CE was preprocedure absolute CD34+ cells. Though the mean CE2 was higher than CE1, it was not statistically significant.
ISSN:0973-6247
1998-3565
DOI:10.4103/0973-6247.164273