Derivation of a MUSIi012-A iPSCs from mobilized peripheral blood stem cells

CD34+ cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the pluripotent phenotype, the ability to differe...

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Bibliographic Details
Published in:Stem cell research 2019-12, Vol.41, p.101597-101597, Article 101597
Main Authors: Laowtammathron, Chuti, Srisook, Pimonwan, Chingsuwanrote, Pimjai, Jiamvoraphong, Nittaya, Waeteekul, Supaporn, Terbto, Papussorn, U-Pratya, Yaowalak, Lorthongpanich, Chanchao, Issaragrisil, Surapol
Format: Article
Language:English
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Summary:CD34+ cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers, and a normal karyotype.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2019.101597